نتایج جستجو برای: fetal calf serum

تعداد نتایج: 393466  

Journal: :Biochemical and biophysical research communications 2001
S Sharmin K Sakata K Kashiwagi S Ueda S Iwasaki A Shirahata K Igarashi

The toxicity of extracellular spermine, determined in the presence of fetal calf serum, was studied using three cell lines: FM3A, L1210, and NIH3T3 cells. Amine oxidase in fetal calf serum produces aminodialdehyde generating acrolein spontaneously, H(2)O(2), and ammonia from spermine. Spermine toxicity was prevented by aldehyde dehydrogenase, but not by catalase. Similar concentrations of sperm...

Journal: :Annals of the rheumatic diseases 1992
P M van der Kraan E L Vitters W B van den Berg

The effect of transforming growth factor beta (TGF beta) on proteoglycan synthesis and degradation in anatomically intact articular cartilage of murine patellae was studied. Exogenously added TGF beta up to a concentration of 200 pmol/l had no effect on proteoglycan synthesis in intact articular cartilage. Neutralisation of endogenously produced TGF beta with a specific monoclonal antibody to T...

Journal: :The Journal of pharmacology and experimental therapeutics 2000
J J Moreno

Previous results suggested that the cyclooxygenase-2 pathway and prostaglandins might modulate 3T6 fibroblast growth. This study shows the effect of resveratrol on the main elements of arachidonic acid (AA) cascade and 3T6 fibroblast growth. The polyphenol reduced the reactive oxygen species production stimulated by fetal calf serum or platelet-derived growth factor, as well as phospholipase A(...

Journal: :Infection and immunity 1988
P A Campbell B P Canono J L Cook

Data presented here demonstrate that recombinant gamma interferon (rIFN-gamma) activated a single population of 10% fetal calf serum-elicited mouse peritoneal exudate cells to express tumoricidal activity but not bactericidal activity for the facultative intracellular bacterium Listeria monocytogenes. Fetal calf serum-elicited cells incubated with rIFN-gamma phagocytosed listeriae normally, sug...

Journal: :ALTEX 2018
Jan van der Valk Karen Bieback Christiane Buta Brett Cochrane Wilhelm G Dirks Jianan Fu James J Hickman Christiane Hohensee Roman Kolar Manfred Liebsch Francesca Pistollato Markus Schulz Daniel Thieme Tilo Weber Joachim Wiest Stefan Winkler Gerhard Gstraunthaler

The supplementation of culture medium with fetal bovine serum (FBS, also referred to as "fetal calf serum") is still common practice in cell culture applications. Due to a number of disadvantages in terms of quality and reproducibility of in vitro data, animal welfare concerns, and in light of recent cases of fraudulent marketing, the search for alternatives and the development of serum-free me...

Journal: :The Biochemical journal 1990
Q G Li R Blacher F Esch L F Congote

A 46 kDa heparin-binding protein which inhibits thymidine incorporation in cultures of fetal calf liver erythroid cells was isolated from fetal bovine serum by affinity chromatography on heparin-Sepharose, ion-exchange chromatography, gel filtration and reversed-phase h.p.l.c. The N-terminal sequence of the first 22 amino acids showed 81% identity with the published sequence of human apolipopro...

Journal: :Cancer research 1989
M Decker E S Chiu C Dollbaum A Moiin J Hall R Spendlove M T Longaker R Stern

The sine qua non of malignancy is the ability of tumor cells to migrate and invade surrounding tissue. There are many substances that have been described that enhance cell motility and hyaluronic acid is prominent among these. Hyaluronic acid is a high molecular weight alternating disaccharide polymer found in abundance in extracellular matrices whenever rapid cell proliferation or tissue regen...

Journal: :The Journal of Cell Biology 1972
Peter J. Stambrook Jesse E. Sisken

The rates of uridine-5-(3)H incorporation into RNA and the rates of uridine uptake into the acid-soluble pool during the cell cycle of V79 Chinese hamster cells were examined. Cells cultured on Eagle's minimal essential medium supplemented with fetal calf serum, lactalbumin hydrolysate, glutamine, and trypsin displayed rates of incorporation and uptake which increased only slightly during G(1) ...

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