Measurements are presented of how polymer surface diffusion at the solid-liquid interface is controlled by surface coverage. The method of measurement was fluorescence correlation spectroscopy (FCS), and the system was poly(ethylene oxide) (PEG) adsorbed onto methyl-terminated self-assembled monolayers in buffered aqueous solution. The translational diffusion coefficient at first increased with...

Inverse-Fluorescence Correlation Spectroscopy (iFCS) is a recently developed modification of standard FCS that allows analysis of particles and biomolecules without labeling. The particles generate no signal; instead the signal is generated by a surrounding medium. Particles diffusing through the FCS-detection volume displace a fraction of the surrounding medium, causing transient dips in the d...

The fusion of Time-Correlated Single Photon Counting and Fluorescence Correlation Spectroscopy, called Fluorescence Lifetime Correlation Spectroscopy (FLCS), is a method that uses picosecond time-resolved fluorescence detection for separating different FCS-contributions. The main emphasis is on the word separating. FLCS does not involve fitting of a complicated model equation to a complicated a...

We calculate the contributions of the flavor changing scalar (FCS) couplings arised from topcolor-assisted technicolor (TC2) models at tree-level to the tγ and tZ production at the Tevatron and LHC experiments. We find that the production cross sections are very small at the Tevatron with √ s = 1.96TeV , which is smaller than 5 fb in most of the parameter space of TC2 models. However, the virtu...

The plasma membrane of various mammalian cell types is heterogeneous in structure and may contain microdomains, which can impose constraints on the lateral diffusion of its constituents. Fluorescence correlation spectroscopy (FCS) can be used to investigate the dynamic properties of the plasma membrane of living cells. Very recently, Wawrezinieck et al. (Wawrezinieck, L., H. Rigneault, D. Margu...

The human brain is a highly connected and integrated system. Local stroke lesions can evoke reorganization in multiple functional networks. However, the temporally-evolving patterns in different functional networks after stroke remain unclear. Here, we aimed to investigate the dynamic evolutionary patterns of functional connectivity density (FCD) and strength (FCS) of the brain after subcortica...

Fluorescence Correlation Spectroscopy (FCS) is a very popular method to determine the diffusion coefficient of molecules in solution. However, the information contained in a FCS curve can be further exploited: As the mean number of molecules in the confocal detection volume Veff is inversely proportional to the amplitude of the correlation function at very short correlation times G (τ ≈ 0),...

Batches of commercial fetal bovine serum, described by the suppliers as antibody-free, all contained antibody to bovine syncytial virus (BSV) when tested by indirect immunofluorescence. Antibody to bovine respiratory syncytial virus (RSV) was not detected in these sera. Twenty-four percent of individual fetal bovine sera contained antibody to BSV, and 14% contained antibody to RSV when tested b...

Following suggestions that transferrin present in fetal-bovine serum, a common supplement used in tissue-culture media, may not bind well to human cells, we have isolated the protein and investigated its interaction with both human and bovine cells. Bovine transferrin bound to a human cell line, K562, at 4 degrees C with a kd of 590 nM, whereas human transferrin bound with a kd of 3.57 nM, a 16...

OBJECTIVE To evaluate primary cultures from human conjunctiva supplemented with fetal bovine serum, autologous serum, and platelet-rich autologous serum, over human amniotic membrane and lens anterior capsules. METHODS One-hundred and forty-eight human conjunctiva explants were cultured in CnT50(®) supplemented with 1, 2.5, 5 and 10% fetal bovine serum, autologous serum and platelet-rich auto...