Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step proced...

Abstract: This paper aims to develop a mathematical model to analyze the passenger waiting time in an intermodal station in which intercity transit system served with feeder buses. Passengers are categorized into two groups, named scheduled and random, respectively. The analytic model is developed for quantifying the relationships of passenger waiting time to reliability of feeder bus services ...

nano sulfated titania was tested as solid acid heterogeneous catalyst for the synthesis of 4-(trifluoromethyl) coumarin and coumarin derivatives under solvent-free condition. factorial design evidenced a positive effect of reaction temperature, amount of catalyst, and solvents.  this nano-sized sulfated titania has been prepared by a sol-gel hydrothermal process. the prepared sulfated titania s...

a condensation of various isatins with indole derivatives has been carried out in the presence of 1,3-dibromo-5,5-dimethylhydantoin (dbdmh) as catalyst at 50 °c in ethanol in order to form 3,3-bis(indolyl) oxindoles. the products were obtained within 20-90 min by 70-96% yields. the efficacy of the procedure has been confirmed by catalyzing the condensation reaction of pyrrole which was used as ...

Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with diffe...

Mouse embryonic fibroblasts (MEFs) have been previously used as feeder cells to support the growth of human embryonic stem cells (hESCs). In this study, human adult uterine endometrial cells (hUECs), human adult breast parenchymal cells (hBPCs) and embryonic fibroblasts (hEFs) were tested as feeder cells for supporting the growth of hESCs to prevent the possibility of contamination from animal ...

The rabbit is a classical experimental animal species. A major limitation in using rabbits for biomedical research is the lack of germ-line-competent rabbit embryonic stem cells (rbESCs). We hypothesized that the use of homologous feeder cells and recombinant rabbit leukemia inhibitory factor (rbLIF) might improve the chance in deriving germ-line-competent rbES cells. In the present study, we e...

Ex vivo culture of human limbal epithelial cells (LECs) is used to treat limbal stem cell (LSC) deficiency, a vision loss condition, and suitable culture systems using feeder cells or serum without animal elements have been developed. This study evaluated the use of human umbilical cord or placenta mesenchymal stem cells (C-MSCs or P-MSCs, resp.) as feeder cells in an animal/serum-free cocultur...

Prolonged propagation of human embryonic stem (hES) cells is currently achieved by coculture with primary mouse embryonic fibroblasts (MEFs) serving as feeder cells. Unlike mouse ES cells, adding growth factors such as leukemia inhibitory factor is insufficient to maintain undifferentiated hES cells without feeder cells. The presence of uncharacterized rodent cells or crude extracts imposes a r...