BACKGROUND Pseudoalteromonas haloplanktis is a cold-adapted γ-proteobacterium isolated from Antarctic sea ice. It is characterized by remarkably high growth rates at low temperatures. P. haloplanktis is one of the model organisms of cold-adapted bacteria and has been suggested as an alternative host for the soluble overproduction of heterologous proteins which tend to form inclusion bodies in e...

human granulocyte-colony stimulating factor (hg-csf) cdna was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase (aox1) promoter. an expression vector for hg-csf secretion was constructed using vector ppic9. higher levels of hg-csf was obtained using a p. pastoris mut+ (methanol utilization fast) phenotype. the effects of environmental factors such as...

The current study evaluates the performance of a pilot scale fed batch reactor (FBR), fo llowed by coagulation and sand filtration, for the treatment of an oil refinery wastewater. The total reductions in COD, BOD, oil contents and phenol were 95.11%, 94.30%, 99.47% and 100%, respectively, with FBR followed by coagulation and sand filtration. This FBR-coagulation-sand filtration treatment syste...

A process comprising two-step fed-batch cultivation has been investigated for the production of ectoines using the halophilic bacterium Halomonas boliviensis DSM 15516(T). The first cultivation was performed under optimal conditions for cell growth and resulted in cell mass concentration of about 41 g l(-1) after 24 h of cultivation. During the second cultivation at higher salt concentration, a...

The present study was designed for the production and optimization of the C12-C14 sophorolipid, using the yeast Candida bombicola ATCC-22214. The fermentation was carried under fed-batch culture conditions i.e., maintaining 15% coconut oil and 10% glucose as hydrophobic and hydrophilic carbon sources, respectively. A maximum yield 54.0 g/L (in 234 h) was achieved. A significant antimicrobial ac...

The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XETTechnology. This chemo enzymatic method is useful for biomimetic modification of cellulose surfaces and holds great potential for industrial applicati...

Structural properties of stochastic dynamic programs are essential to understanding the nature of the solutions and in deriving appropriate approximation techniques. This is especially important in the case of multidimensional Markov decision processes where the state space, action space or outcome space are large and optimal solutions become computationally intractable. We concentrate on a cla...

In this study, the potential of four different culture selection methods under short-term enrichment time (STE) to accumulate PHA-producing bacteria in mixed activated sludge was compared and the most efficient culture selection method was introduced. This means, PHA-producing microbial community was firstly enriched in a sequencing batch bioreactor (SBR) with four different selection methods i...