Fed-batch cultures are used in producing monoclonal antibodies industrially. Existing protocols are developed empirically. Model-based tools aiming to improve productivity are useful with model reliability and computational demand being important. Herein, a systematic framework for developing predictive models is presented comprising of model development, global sensitivity analysis, optimal ex...

BACKGROUND Enzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has several other drawbacks such as low rate of reaction. In the present study we have attempted to enhance the concentration of sugars in enzymatic hydrolysate of delignified Prosopi...

Astaxanthin is a main carotenoid pigment. Fed-batch fermentation bioprocesses of astaxanthin in 2.0 M fermentor have been investigated. Moreover, pH shift control was employed in fed-batch fermentation at 2.0 M scale. The results of astaxanthin fermentation showed that the fed-batch fermentation with pH shift strategy was the best among all the experiments, and the maximum astaxanthin concentra...

BACKGROUND The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted...

BACKGROUND To make lignocellulosic fuel ethanol economically competitive with fossil fuels, it is necessary to reduce the production cost. One way to achieve this is by increasing the substrate concentration in the production process, and thus reduce the energy demand in the final distillation of the fermentation broth. However, increased substrate concentration in simultaneous saccharification...

The relationship between lipid content and tolerance to freezing at -50 degrees C was studied in Saccharomyces cerevisiae grown under batch or fed-batch mode and various aeration and temperature conditions. A higher free-sterol-to-phospholipid ratio as well as higher free sterol and phospholipid contents correlated with the superior cryoresistance in dough or in water of the fed-batch-grown com...

An unconventional modelling methodology based on artificial neural networks is proposed to rapidly develop a model from data obtained during different batch experiments. The objective of the global predict time evolution concentrations all species present in reaction medium. For this, recurrent are elaborated estimate particular as function operating parameters and then assembled complex model....

tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. on the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant e. coli, and consequently fed-batch culture have received less attention....