Fasciola spp. were collected from naturally infected cattle at a local abattoir of Khanh Hoa province, Vietnam, for morphological and genetic investigations. Microscopic examination detected no sperm cells in the seminal vesicles, suggesting a parthenogenetic reproduction of the flukes. Analyses of sequences from the first and second internal transcribed spacers (ITS1 and ITS2) of the ribosomal...

An evaluation of lesions in the livers of Black and White cattle, aged 4 to 7, naturally infested with Fasciola hepatica was performed. The studies included a comprehensive analysis of histological lesions and liver enzyme activities determined with histochemical methods depending on the degree of natural Fasciola hepatica infestation. The studies revealed increased number of reticular fibres, ...

The aim of the present work was to investigate the Schistosoma mansoni and Fasciola gigantica cross-reactivity between adult worms and egg homogenates of the parasites. Immunoprophylactic effects of crude Schistosoma mansoni worms and egg antigens mixed with or without saponins extracted from Atriplex nummularia were studied followed by challenge with 80 cercariae of Schistosoma mansoni. Our re...

background: the trematodes of the genus fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. differentiation of fasciola species is important because of their different transmission and epidemio-logical characteristics. the current study was undertaken to discriminate fasciola species in the domestic ruminants of urmia city, iran. methods: adult fluk...

BACKGROUND Fascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analys...

BACKGROUND Fasciolosis in livestock is a crucial concern in the globe, mainly due to its impact on human health. The aim of this study was to determine the rate of infection with Fasciola gigantica (Cobbold, 1855) larvae in the field-collected snails of Lymnaea auricularia (Linnaeus, 1785) from northwestern Iran using a molecular approach. METHODS A total of 6,759 pond snails were collected f...

A total of 52 fecal samples were collected from 21 herbivores at Dhaka zoo during January to May, 2012 to investigate the prevalence of gastrointestinal parasites of herbivores. Overall prevalence of parasitic infection was 76.9%, of which 61.5% were positive for helminths and 55.8% were positive for protozoa. The identified parasites were Balantidium coli (55.8%), Paramphistomum sp. (36.5%), F...

BACKGROUND Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of the genus Fasciola. In many parts of the world, particularly in poor rural areas where animal disease is endemic, the parasite also infects humans. Adult parasites reside in the bile ducts of the host and therefore diagnosis of human fascioliasis is usually achieved by coprological examinations t...

The aim of this study was to identify Fasciola isolates collected from natural livers of livestock by RFLP and sequencing in southwest of Iran. Morphological examination and biologic characters cannot cause a certainty in the accurate and precise identification and intra and inter-specific differences of Fasciola spp. In this study, we identified Fasciola species using 28S marker in isolates fr...

OBJECTIVE(S) Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on I...