BACKGROUND Accurate diagnosis of Clostridium difficile infection (CDI) is paramount for patient management. The wrong diagnosis places patients at risk, delays treatment, and/ or contributes to transmission of infection in the healthcare setting. Although amplification of the toxin B gene by polymerase chain reaction (PCR) is a sensitive method for detecting toxigenic C. difficile, false negati...

We study negative modes around the Coleman-de Luccia bounce solution. The conditions for appearance of an infinite number do not coincide between Lagrangian and Hamiltonian formulations in literature, we discuss origin this difference detail. show how different choices variable fluctuation solution affect mode condition approach, point out that there exists a choice which gives same as approach.