نتایج جستجو برای: fab fragment
تعداد نتایج: 67229 فیلتر نتایج به سال:
Auto-inducible promoter systems have been reported to increase soluble product formation in the periplasm of E. coli compared inducer-dependent systems. In this study, we investigated phosphate (PO4)-sensitive phoA expression system (pAT) for production a recombinant model antigen-binding fragment (Fab) detail. We explored impact non-limiting and limiting PO4 conditions on strain physiology as ...
The in vivo formation and subsequent fate of complexes formed between specific rabbit gammaG-antibody subunits and circulating protein antigens was studied in rabbits and guinea pigs. Subunits obtained from purified antibodies were injected immediately after an injection of antigen, and the elimination from the circulation of either I*-labeled gammaG-subunits or labeled antigen determined. In t...
Protein 315, a mouse myeloma IgA protein which binds nitrophenyl ligands, and its pepsin-produced Fab’ fragment have been purified by affinity chromatography. The Fab’ fragments were found to be homogeneous by polyacrylamide electrophoresis and isoelectric focusing, and to possess a uniform binding constant. These fragments were readily crystallizable at low salt concentration near their isoele...
The murine monoclonal IgG1 antibody 7A9 binds specifically to the endothelial leukocyte adhesion molecule-1 (E-selectin), inhibiting the attachment of neutrophils to endothelial cells. The primary and three-dimensional structures of the Fab fragment of 7A9 are reported. The amino acid sequence was determined by automated Edman degradation analysis of proteolytic fragments of both the heavy and ...
Antibody engineering and advances in microbial expression systems have enabled production of small, active antibody fragments. However, the functional expression yields of these recombinant antibodies vary widely. The results described in this thesis elucidate factors influencing the expression of stable and active antibody fragments in bacteria. The model antibody used throughout this study wa...
OBJECTIVES As an alternative therapeutic approach for the prevention and treatment of bacterial infections with P. aeruginosa of cystic fibrosis (CF) patients, chicken yolk antibodies (IgY) could be used. The most significant advantage of IgY, in contrast to mammalian IgG, consists in the fact, that when bound to the antigen, they usually do not induce inflammatory reaction. In addition, the si...
Recombinant immunotoxins were made by fusing the Fab domain of monoclonal antibody (MAb) B3 to PE38M, a truncated mutant form of Pseudomonas exotoxin (PE). The recombinant toxins were made in Escherichia coli by fusing genes encoding the antibody domains to a gene encoding the mutant form of PE. MAb B3 binds to a carbohydrate antigen found on many kinds of carcinomas. Immunotoxins in which MAb ...
We developed a method to elucidate the complete structure of triacylglycerols by means of high-energy collisional activation tandem mass spectrometry (MS/MS). Both ESI- and FAB-produced [M + NH4]+ and [M + met.]+ ions (where met. = Li, Na, and Cs) of triacylglycerols undergo charge-remote and charge-driven fragmentations. We emphasize the study of fragment ions from ESI-produced [M + NH4]+ and ...
We characterize a monoclonal antibody directed against the antigen/Ia receptor of a cloned helper T cell line that induced T cell clone proliferation and T cell clone-dependent B cell proliferation at antibody concentrations as low as 10(-11) M. A Fab fragment of this antibody was not stimulatory, implicating cross-linking of antigen receptors as the primary signal for T cell activation. The Fa...
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