The objective of this research is to lift the dormancy seeds S. kunthianum in with a view domesticating them. In experiment we used soaking sulfuric acid for different durations (10 min, 30 min and 1 h). treated were sown transparent germinators. explants obtained then transplanted into pots containing five types substrate (black earth, fine sand, sawdust, mixtures 1/1 sawdust/black earth sawdu...

Skin infection studies are often limited by financial and ethical constraints, and alternatives, such as monolayer cell culture, do not reflect many cellular processes limiting their application. For a more functional replacement, 3D skin culture models offer many advantages such as the maintenance of the tissue structure and the cell types present in the host environment. A 3D skin culture mod...

m: Silicone/fluomsilicone copolymer oii (SiFO) is a polysiloxane derivative potentially us&d as mtraoperative tool and long term retinal tatnponade agent. SiFO is characterized by higher-than-water density and low viscosity; its intraocular tolerance in rabbits up to two months is siroilra to that of silicone oil. &j&t&. SiFO was used in 9 eyes with complicated retinal detachment. Five patients...

Intestinal explants are a useful model for toxicological and pathological studies, but the maintenance of tissue viability for longer periods of incubation remains challenging. The aim of this study was to evaluate the effect of age on the viability of intestinal explants. Jejunal explants were obtained from 24and 42-day-old piglets. The explants were incubated for two or four hours and were th...

We recently described a technique for obtaining neurite outgrowth from explants of larval Xenopus I and adult goldfish retinal As is true of explants from other neural tissues, such as dorsal root ganglion a and spinal cord 12,18, these cultures are comprised of a heterogeneous population of neurons, any of which might give rise to neuritic processes. There was inferential evidence that the neu...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...

present study was conducted to consider the effects of different concentrations of 2,4-d and ba on somatic embryogenesis induction, development and maturation of three strawberry cultivars (kurdistan, parose and camarosa). for this purpose, leaf blade, nodal, petiole, stamen and flower bud calli were cultured on murashige and skoog (ms) medium supplemented with 2, 4 dichlorophenoxyacetic acid (...

A novel protocol for indirect shoot organogenesis of Adhatoda vasica was developed using petiole explants derived from mature shrubby plants. Media with concentrations of cytokinins in combination with auxins were used to induce callus formation in two explants types: petiole and leaf segment. The frequency of callus formation from petiole and leaf segment explants on Murashige and Skoog (MS) b...