Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

The movement of abscisic acid in the cotton explant was investigated by bioassay and radioisotope techniques. The rate of movement was 20 to 30 millimeters per hour with most of the abscisic acid moving unchanged through the explant into the basal agar. The rate of movement was the same through the abscission zone as through petiole tissue. Patterns of accumulation and metabolic products are di...

Mouse submandibular salivary gland cells were grown in primary explant culture. After an initial period of degeneration within the explant, surviving epithelial cells proliferated rapidly and duct-like structures recolonized the explant. Autoradiographic studies showed that a peak of DNA synthesis occurred after 4 days in vitro and that proliferation was enhanced by insulin and hydrocortisone. ...

Effect of Explant, Type and Concentration Hormone on Callus Induction Regeneration two Iranian Sesame (Sesamum indicum L.) Cultivars

OBJECTIVES Clinical trials of spinal cord stimulation (SCS) have largely focused on conversion from trial to permanent SCS and the first years after implant. This study evaluates the association of type of SCS and patient characteristics with longer-term therapy-related explants. MATERIALS AND METHODS Implanting centers in three European countries conducted a retrospective chart review of SCS...

Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

An in vitro steroidogenesis assay using H295R human adenocarcinoma cells has been suggested as a possible alternative to gonad explant assays for use as a Tier I screening assay to detect endocrine active chemicals capable of modulating steroid hormone synthesis. This study is one of the first to investigate the utility of the H295R assay for predicting effects and/or understanding mechanisms o...

AIM To determine the immunohistochemical characteristics of putative corneal epithelial stem cells remaining on limbal explants maintained in culture. METHODS Human limbal explant cultures were generated from 25 residual corneoscleral donor rims following penetrating keratoplasty. Serial sections of these explants were studied using immunohistochemical techniques with a panel of antibodies, o...

Purpose: Rauwolfia serpentina (L.) Benth, from the family, Apocynaceae, is an important medicinal plant due to the alkaloid content of its root. The purpose of this study was to obtain roots directly from leaf explant using growth regulators. Methods: The leaf explant was inoculated on MS (Murashige and Skoog) medium supplemented with single and combinations of growth regulators. Root growth wa...