Cellulose-degrading bacteria were identified from distillery lees, and the strains optimized for fermentation enzyme production, providing effective resource utilization of lees developing cellulase. Based on univariate test, response surface test was used to optimize production conditions fermentation. The screened strain JZ2 had a clear circle-to-colony diameter ratio 2.0. activities exogluca...

CelA is the most abundant enzyme secreted by Caldicellulosiruptor bescii and has been shown to outperform mixtures of commercially available exo- and endoglucanases in vitro. CelA contains both a glycoside hydrolase family 9 endoglucanase and a glycoside hydrolase family 48 exoglucanase known to be synergistic in their activity, connected by three cellulose-binding domains via linker peptides. ...

The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cell...

The aims of this study were the isolation and screening of fungal cultures from forest litter soil for cellulases production. Four fungal cultures were isolated and identified. Among these fungal cultures, three belonged to the genus Aspergillus and one belonged to the genus Penicillium. These fungal cultures were tested to find their ability to produce cellulases, that catalyze the degradation...

In the present investigation, Bambusa bambos was used for optimization of enzymatic pretreatment and saccharification. Maximum enzymatic delignification achieved was 84%, after 8 h of incubation time. Highest reducing sugar yield from enzyme-pretreated Bambusa bambos was 818.01 mg/g dry substrate after 8 h of incubation time at a low cellulase loading (endoglucanase, β-glucosidase, exoglucanase...

Glycoside hydrolase (GH) family 48 is an understudied and increasingly important exoglucanase family found in the majority of bacterial cellulase systems. Moreover, many thermophilic enzyme systems contain GH48 enzymes. Deletion of GH48 enzymes in these microorganisms results in drastic reduction in biomass deconstruction. Surprisingly, given their importance for these microorganisms, GH48s hav...

Screening new Clostridium strains that can efficiently utilize lignocellulose to produce hydrogen is extremely important for dark fermentative production. In this study, a mesophilic hydrogen-producing bacterium, identified as populeti FZ10, was newly isolated from compost acclimated by microcrystalline cellulose. The strain could various cellulosic substrates. performances of production cellul...