Proteins A and G were each labelled with two different europium chelates (p-isothiocyanatophenyl-ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid). Their affinities for IgG from rabbit, goat, horse, sheep, mouse, pig, and rat were then measured using time-resolved fluorescence. Protein G labelled with the second chelate was found to be especially effective in binding to go...

introduction: enlargement of the root canal may potentially affect efficient smear layer (sl) removal. the aim of the present in vitro study was to compare sl removal following canal preparation with two different sizes/tapers by means of scanning electron microscopy (sem). methods and materials: a total of 50 extracted human mandibular premolars were decoronated. the teeth were randomly divide...

Recently, the use of superparamagnetic iron oxide nanoparticles (SPIONS) as a new and promising source of iron in agriculture has been suggested that further investigation is needed before extensive field use. In a greenhouse experiment, the effect of coated magnetite nanoparticles with humic acid (Fe3O4/HA NPs) was investigated on iron deficiency chlorosis and photosynthesis efficiency compare...

The absorption of the nonionic contrast medium iohexol, the clearance tracer 51Cr-ethylenediaminetetraacetic acid, and the blood-pool marker 125I-human serum albumin was studied after subarachnoid and subdural injection in rabbits. Subdural deposition of the contrast medium and 51Cr-ethylenediaminetetraacetic acid resulted in a faster absorption rate and higher achieved blood levels than a suba...

A 56-yr-old man with hypogonadism, gynecomastia, and mental retardation was evaluated for chromosome constitution and thrombocytopenia. Chromosomal analysis demonstrated the mosaicism of 48, XXYY and 47, XXY in the peripheral lymphocytes. Twenty out of twenty-five cells were 48, XXYY karyotype and the remaining five were 47, XXY karyotype. Thrombocytopenia was the EDTA-dependent pseudothrombocy...

The lysis of certain gram-negative bacteria by ethylenediaminetetraacetic acid (EDTA) and lysozyme was first reported by Repaske (7, 8). Lysis by EDTA alone was reported for Pseudomonas aeruginosa (3, 8), Vibrio succinogenes (9), and Alkaligenes faecalis (3). The mechanism for lysis by EDTA (2) in conjunction with lysozyme has been attributed to the interruption of the integrity of the cell sur...

Interest in ethylenediaminetetraacetic acid (EDTA) arises from its ability to combine with metal ions to form water-soluble chelates. Several important biological applications have been made of this property. The material has been shown to be effective (a) in mobilizing radioactive elements such as Pu, Am, and Y from the skeleton (1, 2), (b) as a valuable therapeutic agent in the treatment of p...

Cultures of chick heart fibroblasts were perfused with the chelating agent ethylenediaminetetraacetic acid (EDTA). Cellular responses were observed under phase optics and recorded by time-lapse cinemicrography. In interphasic fibroblasts, EDTA induces cellular contraction followed by continuous protrusion and retraction of ectoplasmic blebs ("surface bubbling"), formation of motile vermiform pr...