Ethanol production from sugarcane bagasse pith hydrolysate by thermotolerant yeast Kluyveromyces sp. IIPE453 was analyzed using response surface methodology. Variables such as Substrate Concentration, pH, fermentation time and Na2HPO4 concentration were found to influence ethanol production significantly. In a batch fermentation, optimization of key process variables resulted in maximum ethanol...

HE CONCENTRATIONS of endogenous ethanol reported in various tissues were the subject of recent review (1). It was concluded that the available evidence points to a normal level of ethyl alcohol in humans of between 20 and 30 mg. per liter of blood or serum. Such concentrations require the formation of ethanol in amounts equivalent to between 14 and 20 per cent of the basal metabolic rate. Becau...

Direct fermentation of 226 white rose tapioca stem to ethanol by Fusarium oxysporum was studied in a batch reactor. Fermentation of ethanol can be achieved by sequential pretreatment using dilute acid and dilute alkali solutions using 100 mesh tapioca stem particles. The quantitative effects of substrate concentration, pH and temperature on ethanol concentration were optimized using a full fact...

A three year old girl presented in a deeply comatose state. She had drunk ethanol four hours previously and her blood ethanol concentration on arrival was 79.8 mmol/litre (3.69 g/litre). Because of her young age, high blood ethanol concentration, time since ingestion, and severe neurological depression on presentation, nasogastric aspiration of the stomach contents was performed and 4.2 g of et...

There is strong evidence demonstrating that nifedipine dissolved in ethanol selectively inhibits only Ltype Ca current. In addition, acute ethanol exposure reduces voltage-dependent calcium currents. In the present study, we investigated the antagonistic effect of fixed concentration of nifedipine dissolved in different concentration of ethanol on L-type Ca current. In a Na-K free solution, nif...

The effect of ethanol on N-demethylation of aminopyrine in rat liver slices and in the microsomal fraction and on microsomal hydroxylation of pentobarbital and aniline was studied. With liver slices N-demethylation of aminopyrine was stimulated by 35-40% at low ethanol concentrations (2mm), whereas no stimulation occurred at high concentrations (100mm). With the liver microsomal fraction, an in...

We describe a case of ethanol and isopropanol ingestion that resulted in coma. The concentration of ethanol and isopropanol was 0.90 and 1.65 g/liter in serum and 3.12 and 5.34 g/liter in gastric contents. With an enzymatic (alcohol dehydrogenase) method for ethanol determination we obtained erroneous analytical results. Because of partial cross reactivity with isopropanol, ethanol concentratio...

ABSTRACT Mass methanol poisonings present a serious problem for health systems worldwide, with poor outcome associated with delayed treatment. Positive pre-hospital serum ethanol concentration may have predictive value as the prognostic factor of the treatment outcome. We studied the effect of positive serum ethanol level on admission to hospital on survival in patients treated during the Czech...

This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-...

The effect of ethanol on the metabolism of S-warfarin and diclofenac by recombinant cytochrome P450 2C9.1 microsomes (CYP2C9.1) was studied. The 7-hydroxylation metabolism of S-warfarin was inhibited by as low as 0.1 vol% (17 mM) ethanol. Ethanol decreased the V(max)/K(m) and V(max) values of S-warfarin metabolism in a concentration-dependent manner, but the K(m) value was unchanged by ethanol....