G-protein coupled estrogen receptor (GPER) is a transmembrane that mediates non-genomic effects of estrogen. This study aimed to investigate the role GPER in stemness formation and malignancies triple negative breast cancer (TNBC) cells. Spheroids MDA-MB-468 cells were induced by mammosphere culture, proportion CD44+ /CD24−/low stem cell subpopulation was detected. Malignant characteristics, ex...

Estradiol (E2) binding activity was studied in several normal tissues and neoplasias of humans and experimental animals. Positive E2 binding was seen in 37 percent of breast primary malignancies, 50 percent of breast cancer metastasis to other organs, and 55 percent of breast cancer metastasis to lymph nodes. Two other tumors, renal cell carcinomas and thyroid adenocarcinomas, also had a high p...

The natural estrogen 17β-estradiol (E2) is one of the known endocrine disrupting chemicals (EDCs) that have been entering water systems via human and animal waste products. The adsorption kinetics and thermodynamics of nylon membranes used in a filter for the removal of 17β-estradiol (E2) in water were studied. Experimental results showed that a linear adsorption isotherm was applicable over th...

Estrogen is traditionally thought to exert genomic actions through members of the nuclear receptor family. Here, we investigated the rapid nongenomic effects of 17β-estradiol (E2) on tumor necrosis factor α (TNF-α) production following lipopolysaccharide (LPS) stimulation in mouse bone marrow-derived macrophages (BMMs). We found that LPS induced TNF-α production in BMMs via phosphorylation of p...

OBJECTIVES The main objective of the present study was to determine whether low physiological levels of estrogen directly protect cardiac cells against metabolic stress. BACKGROUND The beneficial effect of estrogens on the cardiovascular system has been traditionally ascribed to decrease in peripheral vascular resistance and to an antiatherogenic action. Whether physiological concentrations o...

In this study, we aimed at delineating the possible effect of estradiol (E2) levels on day β-hCG administration ovary by analysingthe Embryo Score (ES) early 2, 44hrs post insemination. Oocyte donor ovarian stimulation protocols were thus used in order to avoid any counteractions E2 with endometrium. All oocytes microinjected and all embryo transfers fresh.E2 E2/oocyte correlated positively ES ...

Aim of the study To examine saliva- and serum concentrations correlation of estradiol (E2) in women undergoing ovarian hyperstimulation for IVF/ICSI. Saliva measurements could simplify stimulation follow up. A 'home' test for E2 could be useful. Methods Prospective interventional academic monocentric study at the Centre for Reproductive Medicine of the University Hospital of Ghent, Belgium. B...

Estrogenic activity of regional water samples was evaluated. Samples obtained from wetlands and ponds involved in various agricultural land uses, from three river sites over four seasons, and from municipal wastewater effluent held in storage lagoons were evaluated. The estrogen-responsive cell line MCF-7 BOS was used in the E-screen assay to determine 17beta-estradiol equivalents (E2 Eq) of wa...

STEPHEN M. ROTH, RICHARD GAJDOSIK AND BRENT C. RUBY. Effects Of Circulating Estradiol On Exercise-Induced Creatine Kinase Activity. JEPonline. 2001;4(2):10-17. The present investigation sought to determine the effects of circulating estradiol (E2) and oral contraceptive use on serum creatine kinase (CK) activity following acute eccentric exercise. Ten college-aged female subjects were grouped a...

Estradiol has direct membrane-mediated effects on neurons and its effects are both neuroprotective and neurotrophic. This hormone modulates brain development and aging and affects neurochemical systems which are affected in age-related cognitive decline, AD and other neuropsychiatric disorders. The aim of the present study was to determine the effect of 17β estradiol (E2) in hippocampus region ...