Chicken Expressed Sequence Tags (ESTs) were analyzed to identify genes associated with myogenesis during embryonic development. A total of 6,184 ESTs were generated from three cDNA libraries constructed from whole embryos (Stage 26), somites associated with neural tube (Stage 15), and limb buds (Stages 21, 24 and 26). Clustering and assembly of 4,998 valid ESTs resulted in 2,329 unique sequence...

Expressed sequence tags (ESTs) from normal and tumor tissues have been deposited in public databases. These ESTs and all mRNA sequences were aligned with the human genome sequence using LEADS, Compugen's alternative splicing modeling platform. We developed a novel computational approach to analyze tissue information of aligned ESTs in order to identify cancer-specific alternative splicing and g...

"Etude etiologique de la circulation ra.pide iutra-rena-le"  Le but de l'angiographle renale n'est pas '.seu­lement le controle anatomique de l'organe, une etude physiologique (Hemodynamique) doit toujours etre prise en consideration.  Le temps normal de la circulation intra-renale selon les differents auteurs varie entre 6 a 8, 8 a 10 et 10 a 12 secondes, mais le temps moyen est de 6 a 8 seco...

Acknowledgments My first days in berlin were actually my first days outside India. That had quite an impact on my ability to get adapted to the new environment. But for that matter, the CMB department of Max Planck Institute was a very good choice. The group members were extremely friendly and easily approachable for both personal as well as work-related issues. To start with the acknowledgment...

We present a computer program, termed V-SAGE (Virtual-SAGE), designed to facilitate the analysis of gene expression profiles by combining elements of SAGE (Serial Analysis of Gene Expression) with high-throughput EST analysis. The program re-iteratively correlates sequence tags adjacent to poly(A) tail sequence strings with a second or several tags located within the cDNA adjacent to the recogn...

We have performed a systematic analysis of gene identification in genomic sequence by similarity search against expressed sequence tags (ESTs) to assess the suitability of this method for automated annotation of the human genome. A BLAST-based strategy was constructed to examine the potential of this approach, and was applied to test sets containing all human genomic sequences longer than 5 kb ...

Untranslated regions (UTR) play important roles in the posttranscriptional regulation of mRNA processing. There is a wealth of UTR-related information to be mined from the rapidly accumulating EST collections. A computational tool, UTR-extender, has been developed to infer UTR sequences from genomically aligned ESTs. It can completely and accurately reconstruct 72% of the 3' UTRs and 15% of the...