Background: Encounter with drug resistance and replacment natural treatment is essential. From olden of Iran and specially Chaharmahal and bakhtiari Province medicinal smokes such as donkey dung & Peganum harmala seed used for treatment of many diseases like Infectious diseases. Regarded to lake of scientific research about antimicrobial effects of this smoke this study was done with aim of com...

The comparative studies of different extracts of the leaves of Parkia biglobosa (Jacq) Benth and Parkia bicolor A. Chev (Mimosaceae) with respect to their photochemical and antimicrobial properties was carried out. Preliminary phytochemical screening showed that both plants had similar constituents namely cardiac glycosides, steroids, tannins and alkaloids. The thin layer chromatography of the ...

FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from consensus on this question. We utilized single-molecule localization microscop...

Five thiourea derivative ligands and their Ni(2+) and Cu(2+) complexes have been synthesized. The compounds were screened for their in vitro anti-bacterial activity using Gram-positive bacteria (two different standard strains of Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pyogenes, Bacillus cereus) and Gram-negative bacteria (Esherichia coli, Pseudomo...

CelR, a protein that regulates transcription of cellulase genes in Thermomonospora fusca (Actinomycetaceae) was purified to homogeneity. A 6-kilobase NotI-SacI fragment of T. fusca DNA containing the celR gene was cloned into Esherichia coli and sequenced. The celR gene encodes a 340-residue polypeptide that is highly homologous to members of the GalR-LacI family of bacterial transcriptional re...

The eukaryotic Kv1 voltage-gated K(+) channel is composed of four alpha subunits and four beta subunits. The full-length beta(2) subunit from Rattus norvegicus has been expressed in Esherichia coli, purified and then crystallized. A careful molecular-replacement study using the structure of the truncated beta(2) subunit reveals that the crystals are perfectly pseudo-merohedrally twinned. While ...

MOTIVATION Many algorithms that integrate multiple functional association networks for predicting gene function construct a composite network as a weighted sum of the individual networks and then use the composite network to predict gene function. The weight assigned to an individual network represents the usefulness of that network in predicting a given gene function. However, because many cat...

Persistence of Vibrio cholerae in waters of fluctuating salinity relies on the capacity of this facultative enteric pathogen to adapt to varying osmotic conditions. In an event of osmotic downshift, osmolytes accumulated inside the bacterium can be quickly released through tension-activated channels. With the newly established procedure of giant spheroplast preparation from V. cholerae, we perf...

LacI gene encodes for the repressor of Lac operon. An Esherichia coli (E. coli) strain with a lacI knockout would yield cells that can constitutively express ß-galactosidase. Use of these cells can considerably reduce the use on an inducer isopropyl-ß-D-1-thiogalactoside (IPTG) and reduce the induction time. Jaeger et al (2) prepared a presumptive lacI knockout in E. coli C29 strain using a λ R...

Background-E.coli is found to be the commonest cause of UTI. However E. coli antibiotic resistance has escalated over the past many years. ObjectiveTo provide an update of prevalence of multidrug resistant E.coli isolates and their antibiotic susceptibility pattern with special reference to northern region of Chandigarh. MethodsThis retrospective study was conducted in the Department of Microbi...