Abstract Many soil microorganisms’ i.e., bacteria and fungi produce secondary metabolites called antibiotics. These are used for the treatment of some bacterial, fungal protozoal diseases humans. There is a need isolation broad spectrum antibiotics from microorganisms due to emergence antibiotic resistance. In present study two producing Klebsiella pneumoniae Bacillus cereus were isolated pharm...

Increased nutrient concentrations have been linked to water quality impairments across the world. In Lake Victoria, second largest freshwater lake in world, decades of eutrophication challenged management and led conditions with compromising levels cyanobacteria cyanotoxins. Our study focuses on Winam Gulf, where a resurgence hyacinth has renewed challenges navigation anoxia, but also may prese...

Transition state analogue boronic acid inhibitors mimicking the structures and interactions of good penicillin substrates for the TEM-1 beta-lactamase of Escherchia coli were designed using graphic analyses based on the enzyme's 1.7 A crystallographic structure. The synthesis of two of these transition state analogues, (1R)-1-phenylacetamido-2-(3-carboxyphenyl)ethylboronic acid (1) and (1R)-1-a...

D-Lysine 5,6-aminomutase from Clostridium sticklandii catalyzes the 1,2-shift of the epsilon-amino group of D-lysine and reverse migration of C5(H). The two genes encoding 5,6-aminomutase have been cloned, sequenced, and expressed in Escherchia coli. They are adjacent on the Clostridial chromosome and encode polypeptides of 57. 3 and 29.2 kilodaltons. The predicted amino acid sequence includes ...

RNases play a central role in the metabolism of RNA. Among the many reactions catalyzed by these enzymes are those that separate individual entities of multicomponent transcripts, those that generate the mature 5' and 3' termini of RNAs, and those that remove introns. RNases also degrade RNA molecules that are no longer needed by the cell or that are damaged in some way, and under some conditio...

Yield of protein per translated mRNA may vary by four orders of magnitude. Many studies analyzed the influence of mRNA features on the translation yield. However, a detailed understanding of how mRNA sequence determines its propensity to be translated is still missing. Here, we constructed a set of reporter plasmid libraries encoding CER fluorescent protein preceded by randomized 5΄ untranslate...

MOTIVATION The operon structure of the prokaryotic genome is a critical input for the reconstruction of regulatory networks at the whole genome level. As experimental methods for the detection of operons are difficult and time-consuming, efforts are being put into developing computational methods that can use available biological information to predict operons. METHOD A genetic algorithm is d...

─ Acute urinary tract infections consist of upper urinary tract infections, including pyelonephritis, prostatitis, intra-kidney abscesses, and around-kidney abscesses, and lower urinary tract infections, including urethra infection and bladder infection. These infections can independently or in combination result in irritation in various parts. They may appear as asymptomatic or as one of the c...

Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal secretory and inflammatory responses to STEC in the bovine intestine, serotyp...