We examined the ultrastructure of Plasmodium malariae-infected erythrocytes in peripheral blood and tissue biopsies of the liver, stomach, and duodenum from three patients infected with P. malariae. Ultrastructural features of P. malariae-infected erythrocytes in peripheral blood appear similar to those described previously. The surface membranes of P. malariae-infected erythrocytes had numerou...

Plasmodium falciparum invades erythrocytes through multiple ligand-receptor interactions, with redundancies in each pathway. One such alternate pathway is the trypsin-resistant pathway that enables P. falciparum to invade trypsin-treated erythrocytes. Previous studies have shown that this trypsin-resistant pathway is dependent on glycophorin B, as P. falciparum strains invade trypsin-digested g...

Glutathione is an important thiol-containing compound involved in the detoxification process in erythrocytes. Its thiol group reacts with a variety of xenobiotics in a glutathione S-transferase catalyzed reaction to form conjugates that are effluxed from the erythrocytes by an ATP dependent transport mechanism. A well studied experimental system is the transport of the conjugate of glutathione ...

BACKGROUND Normal erythrocyte is deformable and this facilitates blood flow in the capillaries. Oxidative stress reduces the deformability of erythrocytes, and influences on blood flow in microcirculation. The objective of this study was to investigate the deformability of erythrocytes exposed to oxidative stress, the protective effects of verapamil and ascorbic acid against oxidative damages i...

The modification of the standard rubella haemagglutination-inhibition (HAI) test using trypsin-treated human group O erythrocytes instead of chick erythrocytes was evaluated. In a comparative study we found that, of 816 samples tested by both methods, the titres of 807 (98.9%) sera were in close agreement within an acceptable twofold difference. Trypsin-treated human group O erythrocytes usuall...

Resealed Erythrocytes are of appropriate size and shape to carry drugs. They are biocompatible and they have minimum toxic side effects. Minimum leakage can be observed before they reach the target site and they are able to carry broad spectrum of drugs. Carrier erythrocytes and resealed erythrocytes are being used for continuous implementation of safe and effective delivery of various drugs fo...

Erythrocytes containing pig liver uricase have been prepared by hypotonic hemolysis in the presence of the enzyme. Uricase is shown to be active within the erythrocytes and to degrade uric acid as rapidly as it enters the cells when high intracellular enzyme concentrations are employed. The kinetics and characteristics of uric acid entry are shown to be the same for hemolysed and normal erythro...

Erythrocyte polymorphisms associated with a survival advantage to Plasmodium falciparum infection have undergone positive selection. There is a predominance of blood group O in malaria-endemic regions, and several lines of evidence suggest that ABO blood groups may influence the outcome of P. falciparum infection. Based on the hypothesis that enhanced innate clearance of infected polymorphic er...

BACKGROUND Hemoglobin C differs from normal hemoglobin A by a glutamate-to-lysine substitution at position 6 of beta globin and is oxidatively unstable. Compared to homozygous AA erythrocytes, homozygous CC erythrocytes contain higher levels of membrane-associated hemichromes and more extensively clustered band 3 proteins. These findings suggest that CC erythrocytes have a different membrane ma...

BACKGROUND Validation of hemagglutination inhibition (HI) assays is important for evaluating antibody responses to influenza virus, and selection of erythrocytes for use in these assays is important. This study aimed to determine the correlation between receptor binding specificity and effectiveness of the HI assay for detecting antibody response to pandemic influenza H1N1 (pH1N1) virus. METH...