The aim of this study was to examine the impact of divalent copper, iron, manganese, and zinc ions on the production of erythritol from glycerol by Yarrowia lipolytica and their effect on the activity of erythrose reductase. No inhibitory effect of the examined minerals on yeast growth was observed in the study. Supplementation with MnSO4 · 7H2O (25 mg l(-1)) increased erythritol production by ...

Klewicka E., Klewicki R. (2009): In vitro fermentation of galactosyl derivatives of polyols by Lactobacillus strains. Czech J. Food Sci., 27: 65–70. Probiotic lactic acid bacteria belonging to the species Lactobacillus casei, Lb. paracasei, and Lb. acidophilus were cultivated in the presence of galactosyl derivatives of erythritol, xylitol, and sorbitol, as well as the polyols themselves: eryth...

The putative catalytic domain (residues 81-401) of a predicted tomato protein with similarity to 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase of Escherichia coli was expressed in a recombinant E. coli strain. The protein was purified to homogeneity and was shown to catalyze the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2-C-methyl-d-erythritol at a rate of 33 mic...

TO THE EDITOR: To Drs. Bonnema and colleagues, thank you for your comments on our recent article in AJP-Endocrinology and Metabolism (4). Indeed, for subjects not used to these polyols, 50 g of xylitol and 75 g of erythritol as a single bolus given within 2 min seems rather high. From a nutritional point of view the applied doses makes sense, at least in adapted subjects. This would then, for i...

Remineralization of minor enamel defects is a normal physiological process that is well known to clinicians and researchers in dentistry and oral biology. This process can be facilitated by various dietary and oral hygiene procedures and may also concern dentin caries lesions. Dental caries is reversible if detected and treated sufficiently early. Habitual use of xylitol, a sugar alcohol of the...

A hypothetical gene with similarity to the ispD gene of Escherichia coli was cloned from Arabidopsis thaliana cDNA. The ORF of 909 bp specifies a protein of 302 amino acid residues. The cognate chromosomal gene consists of 2,071 bp and comprises 11 introns with a size range of 78-202 bp. A fragment comprising amino acid residues 76-302 was expressed in a recombinant E. coli strain. The protein ...

Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the responsible genes has shown a lethal phenotype for Escherichia coli. In the ME...

WHEELERJENRY ~.,ELIZABETH D.Ross, AND STANLEY E. BRADLEY. Canalicular bile production in dogs. Am. J. Physiol. 2 14(4) : 866-874. 1968.-Biliary clearances of 14C-labeled creatinine, erythritol, inulin, mannitol, sucrose, and urea were measured in three unanesthetized cholecystectomized dogs equipped with Thomas duodenal cannulas. Sodium taurocholate was infused intravenously at varying rates to...

The effects of DMSO, ethylene glycol, glycerol, erythritol, dimethylformamide and sucrose on the survival of unfrozen and frozen mouse morulae were examined. All the agents had a deleterious effect on survival of the unfrozen morulae at 20 degrees C. The harmful effect of erythritol was lower at 0 degrees C and sucrose had a protective effect at that temperature. Dimethylformamide and DMSO were...

Isoprenoid precursors, which are a large group of natural products and play key roles in many biological pathways, can only be biosynthesized by the 2-C-methyl-d-erythritol 4-phosphate pathway in Mycobacterium tuberculosis. The 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase (IspE), which is an essential enzyme in the isoprenoid precursor biosynthesis pathway, catalyzes ATP-dependent phosphor...