Abstract Introduction Loss of pregnancy in mares is a major cause economic and emotional impact for horse breeders. It can have many different infectious noninfectious causes. The aim this study was identification the main viral causes abortion Poland based on tissue samples from 180 aborted foetuses submitted testing between 1999 2022. Material Methods Tissues collected studs throughout were t...

The detection and quantification of equine cytokines has been hampered by the lack of antibodies for many years. With the development of antibody pairs for equine cytokines during the past years, the quantification of these essential regulators of the immune response became possible. After being successfully tested by enzyme-linked immunosorbent assays (ELISA), three of these anti-cytokine reag...

Equine herpesvirus type 4 (EHV-4) is one of the most important pathogens in horses. To clarify the key genes of the EHV-4 genome that cause abortion in female horses, we determined the whole genome sequences of a laboratory strain and 7 Japanese EHV-4 isolates that were isolated from 2 aborted fetuses and nasal swabs of 5 horses with respiratory disease. The full genome sequences and predicted ...

Background: Incidental findings of virus-like particles were identified following electron microscopy of tissue-engineered tendon constructs (TETC) derived from equine tenocytes. We set out to determine the nature of these particles, as there are few studies which identify virus in tendons per se, and their presence could have implications for tissue-engineering using allogenic grafts. Methods:...

Equine herpesvirus 1 replicated in the brains of 2-week-old mice but did not produce fatal encephalitis; it thus simulated the majority of cases of herpes simplex encephalitis in man. This replication was not inhibited by iododeoxyuridine, although in tissue cultures the equine and human viruses were equally susceptible. The continued use of iododeoxyuridine for human encephalitis should be ser...

Equid herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaherpesviruses, but genetically, EHV-3 is significantly more divergent.

Latent equine herpesvirus type 1 (EHV-1) infection is common in horse populations worldwide and estimated to reach a prevalence nearing 90% in some areas. The virus causes acute outbreaks of disease that are characterized by abortion and sporadic cases of myeloencephalopathy (EHM), both severe threats to equine facilities. Different strains vary in their abortigenic and neuropathogenic potentia...

The gel diffusion test was applied direct to field samples from cases of equine abortion, foal mortality and equine paralysis due to equine herpesvirus type 1 (EHV-1). Following polyethylene glycol concentra­ tion of viral antigen, one line was formed against EHV-1 antiserum after 24 hours incubation at 25°C. Tests carried out simultaneously on 41 field samples showed a high correlation between...

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-...