The nuclear histones of control and antigen-affected lymphocytes and thymocytes were measured in terms of their intensity of staining with ammoniacal-silver (A-S) and alkaline fast green (FG). Subcutaneous injection of full strength and doubly concentrated tetanus toxoid into the upper forelimb was followed by an acute decrease in the intensity of the FG and A-S staining of the thymocyte nuclea...

We studied 23 cases of angioimmunoblastic lymphadenopathy (AILD) and AILD-like lymphoma for evidence of EpsteinBarr virus (EBV) using the polymerase chain reaction (PCR) and in situ hybridization studies. EBV nucleic acid sequences were found by either PCR or in situ hybridization in 96% of the cases. There was a wide range in the number of EBVpositive cells among the different cases as detecte...

disease, and in other lymphomas. Patients with leukemia and lymphoma, but not carcinomas, also reacted with extracts of tumor-derived lymphoid cell lines. Studies of in vitro cell-mediated cytotoxicity against autologous tumor cells, using the 51Cr release assay, also gave evidence for reactivity against tumor-associated antigens. In humoral and cell-mediated cytotoxicity assays against lymphoi...

Adeno-associated virus (AAV) vectors are attractive for gene delivery-based therapeutics, but data from recent clinical trials have indicated that AAV capsids induce a cytotoxic T lymphocyte (CTL) response that eliminates transduced cells. In this study, we used traditional pharmacological agents and AAV mutants to elucidate the pathway of capsid cross-presentation in AAV-permissive cells. Endo...

A soluble nuclear antigen that reacts with sera obtained from patients with systemic lupus erythematosus and Sjögren's syndrome has been described. The antigen, tentatively named the Ha antigen after the prototype serum, was shown to react with specific antibodies by precipitin, complement fixation, and immunofluorescence techniques. The Ha antigen prepared from isolated nuclei of calf thymus g...