We have optimized the magnetic alignment of phospholipid bilayered micelles (bicelles) for EPR spectroscopy, by varying lipid composition and temperature. Bicelles have been extensively used in NMR spectroscopy for several decades, in order to obtain aligned samples in a near-native membrane environment and take advantage of the intrinsic sensitivity of magnetic resonance to molecular orientati...

The high-spin Fe~III!-center of oxidized rubredoxin from Clostridium pasteurianum shows a complicated, temperature-dependent EPR spectrum. We combine conventional EPR spectroscopy with optically detected EPR ~ODEPR! to elucidate the electronic structure of this protein metal center. The ODEPR experiment, which can be considered as coherent Raman scattering or modulated magnetic circular dichroi...

The major advancement outlined in this communication is the simultaneous visualisation of two kinds of nitroxyl radicals using electron paramagnetic resonance (EPR) spectroscopy and imaging.

Paramagnetic properties of model humic substances (HS) synthesized from 3,4,5-trihydroxybenzoic acid (gallic acid) were studied by means of EPR spectroscopy. A singlet EPR signal without any fine structure was observed for powder sample with spin density 6×10 spin/g, witdh 6.45 Gs and g=2.002. Results of the microwave power saturation pointed to a high dipolar relaxation enhancement. In aqueous...

EPR tooth in vivo dosimetry is an attractive approach for initial triage after unexpected nuclear events. An X-band cylindrical TM010 mode resonant cavity was developed for in vivo tooth dosimetry and used in EPR applications for the first time. The cavity had a trapezoidal measuring aperture at the exact position of the cavity's cylindrical wall where strong microwave magnetic field H1 concent...

A combination of molecular mechanics (MM), electron paramagnetic resonance spectroscopy (EPR), and spectra simulation (MM-EPR) has been used to determine the solution structures of di- and trinuclear copper(II) complexes of melamine-based oligomacrocyclic ligands. The spin Hamiltonian parameters of the mononuclear, melamine-appended macrocyclic ligand copper(II) complex have been determined by ...

The combined use of selected nitroxides and EPR spectroscopy has been proved to be suitable for studying the partitioning rate of a given substrate in cyclodextrin-micelle systems.

EPR spectroscopy and spin-trapping experiments showed that polymer-encapsulated Au nanoparticles promote aldehyde oxidation via a radical pathway by initiating formation of acyl radicals.

Rapid freeze-quench (RFQ) in combination with electron paramagnetic resonance (EPR) spectroscopy at X-band is a proven technique to trap and characterize paramagnetic intermediates of biochemical reactions. Preparation of suitable samples is still cumbersome, despite many attempts to remedy this problem, and limits the wide applicability of RFQ EPR. We present a method, which improves the colle...

Intrinsically disordered proteins (IDPs) form a unique protein category characterized by the absence of a well-defined structure and by remarkable conformational flexibility. Electron Paramagnetic Resonance (EPR) spectroscopy combined with site-directed spin labeling (SDSL) is amongst the most suitable methods to unravel their structure and dynamics. This review summarizes the tremendous method...