Dorsal closure (DC) is an essential step during Drosophila development whereby a hole is sealed in the dorsal epidermis and serves as a model for cell sheet morphogenesis and wound healing. It involves the orchestrated interplay of transcriptional networks and dynamic regulation of cell machinery to bring about shape changes, mechanical forces, and emergent properties. Here we provide insight i...

Experiments are described which provide a direct measure of the adhesion between dermis and epidermis during the development of feather primordia in chick dorsal skin in culture. The epidermis was peeled from the dermis and the surfaces so exposed were examined under the scanning electron microscope: regions of strong adhesion between the tissues were revealed as areas where their separation wa...

The dermal-epidermal recombination technique was used to determine the site of action of the naked (N) locus in the skin of the mouse. The skin of athymic (nude) mice was used as a host site for growth of recombined epidermis and dermis from 13- and 14-day N/+ and +/+ embryos. Grafts that contained mutant epidermis lost their hair by 26 days after grafting (at the end of the first hair cycle) a...

Lrig1 is known to repress the epidermal growth through its inhibitory activity on EGFR, while CD44 promotes it. We analyzed the expression of these molecules in senescent atrophic human epidermis and in the epidermis of CD44KO mice. In normal human epidermis, Lrig1+ cells form clusters located in the basal layer in which CD44 expression is downregulated and Lef1 expression reflects an active Wn...

We have examined integrin expression during the remodeling of the epidermis that takes place during wound healing, using a suction blister model in which the epidermis is detached from the dermis, leaving the basement membrane intact. By immunofluorescence microscopy, we found that the same integrin subunits were expressed during wound healing as in normal epidermis with very little change in t...

plastic epidermis and in lesions of squamous cell carcinoma. The growth fraction in control unirradiated mice was also 100%. The duration of TV and S decreased progressively with induction and progression of the tumor. For normalappearing epidermis of irradiated mice and epidermis of control mice, TVand S were approximately 95 and 6.2 hr, respectively. With the induction of hyperplasia, TV and ...

R A Y L A N K E S T E R (1873) coined the term 'Triploblastic' and supposed that the gut, parenchyma and epidermis of Turbellaria corresponded to the germ layers of contemporary dogma. This idea is still current, though neither the origin, nor the maintenance of the epidermis of planarians has been investigated in detail. Most embryological studies have been restricted to early development, but...

The purpose of the major cancer research project of Barnard Hospital is to measure the properties of epidermis during epidermal carcinogenesis induced by methylcholanthrene, to integrate the findings and thus to construct a comprehensive account of what .happens (6). Mitotic frequency is one of these properties and has been investigated by Cooper and Relier (5) and by Reller and Cooper (12). Th...

Mammalian epidermis is maintained by stem cells that have the ability to self-renew and generate daughter cells that differentiate along the lineages of the hair follicles, interfollicular epidermis and sebaceous gland. As stem cells divide infrequently in adult mouse epidermis, they can be visualised as DNA label-retaining cells (LRC). With whole-mount labelling, we can examine large areas of ...