This study investigated the effectiveness of immobilizing Saccharomyces cerevisiae invertase (SInv) on magnetite nanoparticles to produce fructooligosaccharides (FOSs). Based existing literature and accompanied by parameter estimation, a modified kinetic model was employed represent kinetics sucrose hydrolysis transfructosylation using SInv immobilized nanoparticle surfaces. utilized simulate p...

An enzyme-catalyzed microfluidic assay using magnetic micro-beads is described. Here, diaphorase (DI) (E.C. 1.6.99) is covalently attached to the magnetic micro-beads (2.7 mum) and integrated into a short section of a microchip fabricated from PDMS. DI converts non-fluorescent resazurin to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH). In this work,...

A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried...

A method for immobilization of β-galactosidase from Kluyveromyces lactis on an adsorptive membrane for the continuous synthesis of galacto-oligosaccharides from lactose was carried out. The immobilization was performed at 4, 10, 15 and 40°C. Two strongly basic anion exchange membranes with tradename MustangQ and SartobindQ were investigated. In static experiments, the highest enzyme activity wa...

Immobilized-enzyme reactors are beginning to be studied for a variety of therapeutic applications. To facilitate the design of these devices for different clinical situations and a diverse patient population, mathematical models may be valuable. An immobilized-heparinase (EC 4.2.2.7) reactor was selected as a model system. The device removes heparin from blood that has been anticoagulated to pr...

The catalytic combustion of methane/air mixtures is investigated experimentally and numerically at gas turbine relevant conditions (inlet temperatures up to 873 K, pressures up to 15 bar and spatial velocities up to 3 × 106 h−1). Experiments are performed in a sub-scale test rig, consisting of a metallic honeycomb structure with alternately coated (Pd-based catalyst) channels. Simulations are c...

The photocatalytic oxidation of phenol in water in the band reactor with supported TiO2 catalysts was investigated. Modified anatase/Al2O3 catalysts were used as the photocatalysts. The main aim of these studies was to determine catalytic activity of TiO2/Al2O3 and V-TiO2/Al2O3 in the removal of phenol from water. The effectiveness was measured in the configurations: TiO2/UV/O2/H2O2, TiO2/O2/H2...

Mathematical models, especially when coupled with modern computer techniques, prove to be very effective in searching for optimal operating conditions and creating an optimal microenvironment for the biocatalyst in order to optimise productivity. Therefore, the study of the theoretical model for the enzyme reaction system is of interest for the industrial application of the biocatalyst. Theoret...