We developed a nanofluidics-based enzyme-linked immunosorbent assay (ELISA) device and realized quantification of countable number of molecules by combining enzymatic amplification reaction and ultrasensitive detection with a differential interference contrast thermal lens microscope (DIC-TLM). Our method will contribute to single cell analysis field, in particular for ultrasensitive protein co...

Fifty-three cerebrospinal fluid specimens from meningitis patients were investigated by enzyme-linked immunosorbent assay for detection of mycobacterial antigens. After heating at 56 degrees C for 1 hour to eliminate nonspecific interference, all 22 tuberculous meningitis (TBM) specimens had an optical density of greater than 0.05 (sensitivity 100%). Six out of 31 non-TBM cases gave false-posit...

We describe a multiprobe colorimeter that permits the reading of enzyme-linked immunosorbent assays (ELISA) in situ, by use of microhemagglutination plates. The use of dipping probes eliminates interference from meniscus effects and air-bubble entrapment. Detailed validation tests have been done and potential sources of inaccuracy identified.

The vulnerability of civil GPS receiver to interference may be intentional or unintentional. Among all types of interference, replay attack intended as the most dangerous intentional one. The signal structure of replay attack is almost the same with the satellite signal. The interference effects can be reduce with the design of an appropriate filter in the receiver. This paper presents two meth...

the modeling investigation in this paper discusses the system level effects of a toxicant on a three species food chain system. in the models, we have assumed that the presence of top predator reduces the predatory ability of the intermediate predator. the stability analysis of the models is carried out and the sufficient conditions for the existence and extinction of the populations under the ...

The first enzyme-based biosensors capable of determining glycolic acid in various complex matrixes, such as cosmetics, instant coffee, and urine, are presented in this paper. Two separate designs, both based on a three-membrane configuration consisting of an inner cellulose acetate membrane (CA) and an outer polycarbonate membrane (PC), which sandwich a membrane bearing the biomolecule(s), are ...

Concentrated and partially purified preparations of streptococcal supernates containing streptolysin O have been shown to inhibit selectively the oxidative metabolism of mitochondria procured from the myocardium of rabbits and guinea pigs, the findings pointing to an interference with the action of the coenzyme diphosphopyridine nucleotide (DPN) (1). Further studies have demonstrated that these...

Skin whitening products are commercially available for cosmetic purposes in order to obtain a lighter skin appearance. They are also utilized for clinical treatment of pigmentary disorders such as melasma or postinflammatory hyperpigmentation. Whitening agents act at various levels of melanin production in the skin. Many of them are known as competitive inhibitors of tyrosinase, the key enzyme ...

Exosomes are emerging as one of the most promising biomarkers for early disease diagnosis and prognosis. The significant challenges facing available methods include improving detection specificity sensitivity in complex biological samples. Herein, a fluorescence assay was established based on combination immunomagnetic separation two-step signal amplification strategy direct isolation subsequen...