penicillin g acylase from e. coli ta1 was immobilized by cross-linked enzyme aggregates (clea), a new method for immobilization. this biocatalyst and commercial immobilized penicillin g acylase (pga-450) were used to study the effect of ph, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (pgme) and 6-aminopenicillanic acid (6-apa). compare...

protein hydro-lysate was prepared from gold striped sardine (sardinella gibossa) by papain. hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (rsm) using a factorial design and anti-oxidative activity was the dependent variable. results of the total determination coefficient, adjusted r2 and predicted r2 indicating the validity of pred...

Microporous glass ceramic with skeleton of CaTi4(PO¬4)6 with average pore size of 12.7 nm has been synthesized and used as a carrier of glucose oxidase. The glass ceramic was prepared by controlled heat treatment of glass samples, which causes the phase separation in their structure and creates CaTi4(PO¬4)6 and β-Ca3(PO4)2 phases. The β-Ca3(PO4)2 phase was dissolved by soaking the glass ceramic...

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...

abstract in this study a greenhouse experiment was conducted to evaluate the role of acc deaminase enzyme on alleviation of salinity stress of canola plant. canola plants were exposed to pseudomonas fluorescens strain p12 which contains a rhizobacter capable of producing pseudomonas fluorescens in comparison with plants exposed to mutant pm12 unable to produce the required enzyme. the mutant wa...