High rainfall with increase in humidity (68% to 75%) along a decrease temperature ( 3 5°C less than usual ) during June, July, and August months favor the emergence of white grub beetles, its egg laying which turn resulted severe sugarcane clump damage by grubs September months. Seasonal incidence growing areas Andhra Pradesh state showed that clumps caused infestation was significantly high ma...

The tobacco hornworm, Manduca sexta, is a model lepidopteran insect used to study the pathogenic and mutualistic phases of entomopathogenic nematodes (EPNs) and their bacterial symbionts. While intestinal microbial communities could potentially compete with the EPN and its bacterial partner for nutrient resources of the insect, the microbial gut community had not been characterized previously. ...

We present the annotation of the draft genome sequence of Oscheius sp. TEL-2014 (Genbank accession number KM492926). This entomopathogenic nematode was isolated from grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Oscheius sp. Strain TEL has a genome size of 110,599,558 bp and a GC content of 42.24%. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the...

In a laboratory experiment an efficacy of entomopathogenic nematode Steinernema feltiae in controlling third-stage larvae of common cockchafer (Melolontha melolontha) was studied. The experiment comprised of commercial product Entonem and indigenous strain C76. The efficacy of both biological agents was tested at 20 and 25 oC and at four different concentrations of nematode suspension: 0, 250.0...

Rifampin resistant (Rif(R)) mutants of the insect pathogenic bacterium Photorhabdus luminescens LN2 from entomopathogenic nematode Heterorhabditis indica LN2 were genetically and proteomically characterized. The Rif(R) mutants showed typical phase one characters of Photorhabdus bacteria, and insecticidal activity against Galleria mellonella larvae, but surprisingly influenced their nematicidal ...

Tritrophic interactions involving plants, herbivores and parasites have been only recently documented for belowground systems, where entomopathogenic nematodes can exploit root herbivore induced volatile compounds to locate their hosts. Little is known, however, about whether the specificity of such interactions rivals that of the remarkable interactions found in aboveground studies. Using a be...

Entomopathogenic nematodes are used for biological control of insect pests. A method for improved cryopreservation of infective juvenile stage nematodes has been developed using Steinernema carpocapsae and Heterorhabditis bacteriophora. Optimum survival for both species was achieved with 12,000 infective juveniles/ml in glycerol and 7,500/ml in Ringer's solution. For S. carpocapsae, maximum sur...

In 2009, three Slovenian strains of entomopathogenic nematodes) and commercial product Entonem (active ingredient S. feltiae), were tested under laboratory conditions for their activity against adult cereal leaf beetles (Oulema melanopus). The nematode strains were tested at four different doses (250, 500, 1000, and 2000 infective juveniles/adult) and at three temperatures (15, 20, and 25 oC). ...