Escherichia coli shows severalfold less susceptibility to tetracyclines when grown in enriched medium than in minimal medium. Transport studies with cells harvested from these media showed different handling of the drugs. Whereas an energy-dependent uptake of tetracycline and minocycline was observed in susceptible K-12 and wild-type E. coli strains grown in minimal medium, an active efflux of ...

OBJECTIVES Artesunate, a derivative of dihydroartemisinin, itself a product of artemisinin, inhibits the replication of cytomegalovirus in vitro. In vivo, artesunate undergoes rapid conversion into the active metabolite dihydroartemisinin. The in vitro stability of the compounds and the antiviral activity of dihydroartemisinin are of great concern for the interpretation of in vitro testing. The...

Background Chylothorax is defined as abnormal accumulation of lymphatic fluid in the pleural space secondary to leakage from thoracic duct or one of its main tributaries. Congenital idiopathic chylothorax is the most common form of pleural effusion in neonates. Conservative therapy is the mainstay of treatment. An alternative...

Antigens for Mycoplasma gallisepticum were prepared from organisms propagated in media with and without dextrose supplementation. The antigens made from organisms produced in medium enriched with dextrose were less sensitive than the others in slide agglutination tests.

Mycobacterium Zepraemurium multiplies in a cell-free liquid medium, referred to as NC-5, which is enriched Kirchner medium plus goat serum, a-ketoglutaric acid, cytochrome c, haemin, and L-cysteine. At 30 "C, the bacilli gradually elongated before multiplying 100to Iooo-fold. The maximum number of bacilli is reached after 8 weeks' incubation. The generation time of Mycobacterium lepraemurium is...

genus leishmania is a protozoan parasite that causes different severe diseases. fetal calf serum (fcs) is the major part of the leishmania culture media, for mass cultivation and the most expensive ingredient in these media. in the present work, the efficacy of chicken serum was evaluated in leishmania culture media. the results indicated that, the (10%) chicken serum enriched culture medium su...

1. The effect of vitamin B complex (vitamin B1, B6 and B12) was studied on nerve conduction velocity in acrylamide-neuropathy rats maintained on refined semisynthetic complete vitamin and vitamin B-deficient diets in vivo and on neurite outgrowth in vitro using cells obtained from dorsal root ganglions of mice. 2. Acrylamide neuropathy was clearer in the group maintained on a refined semisynthe...

Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubation of Fu5-AH rat hepatoma cells in medium containing cholesterol-enriched lipop...