PURPOSE Ethacrynic acid (ECA) is a potential trabecular meshwork (TM) drug that has shown promising results in preclinical studies for treatment of primary open-angle glaucoma. However, topical application of ECA is currently limited by adverse effects in corneal tissues. To this end, we developed a new theoretical model to evaluate time-dependent toxicity induced by ECA in corneal epithelial c...

Many microalgae induce an extracellular carbonic anhydrase (eCA), associated with the cell surface, at low carbon dioxide (CO2) concentrations. This enzyme is thought to aid inorganic carbon uptake by generating CO2 at the cell surface, but alternative roles have been proposed. We developed a new approach to quantify eCA activity in which a reaction-diffusion model is fit to data on (18)O remov...

Sterile tissues of human subjects free from infection and malignancy were assessed for an antigen that cross-reacts with the enterobacterial common antigen (ECA). Extracts of heated homogenates (HE) and ethanol-soluble (ES) fractions of liver, kidney, muscle, and heart tissues were examined for their capacities to react with ECA antibodies, as measured by hemagglutination procedures, and to eli...

Event clock automata (ECA) are a model for timed languages that has been introduced by Alur, Fix and Henzinger as an alternative to timed automata, with better theoretical properties (for instance, ECA are determinizable while timed automata are not). In this paper, we revisit and extend the theory of ECA. We first prove that no finite time abstract language equivalence exists for ECA, thereby ...

We investigated the effects of stathmin 1 (STMN1) silencing by small interfering (siRNA) on the sensitivity of esophageal cancer cells Eca-109 to paclitaxel. STMN1 siRNA was transiently transfected into Eca-109 cells. The effects of transfection were detected by quantitative polymerase chain reaction and western blotting. The effects of STMN1 silencing by siRNA on the sensitivity of esophageal ...

Sustainability has become an increasingly important component of requirements for the rehabilitation bridge structures. Responsible use our limited natural resources is essential future generations, so whole process must be taken into account, in terms structural integrity. The environmental impact these types structures major – demolition and reconsideration e.g. reinforced concrete structure ...

BACKGROUND In order to gain insight into neuroprotective effects of ECa 233, a standardized extract of Centella asiatica, previously demonstrated in animal models of memory impairment induced by transient global ischemia or intracerebroventricular injection of β-amyloid, the effect of ECa 233 on neurite outgrowth of human IMR-32 neuroblastoma cell line was investigated. METHODS Cells were see...