The effects of vitrification and cryostorage length in liquid nitrogen (LN2) on in vitro survival of rabbit embryos were examined. A total of 374 morphological normal embryos collected from 20 superovulated multiparous New Zealand White rabbit were divided into two groups. The first group (n=94) was non-vitrified used as a fresh control group and the second group (n=280) was vitrified and stora...

BACKGROUND Assisted hatching (AH) in fresh embryo transfer (ET) cycles increases the implantation and pregnancy rates, especially in women with a poor prognosis, repeated implantation failures and in older women. Little information exists in the literature regarding the role of AH in frozen-thawed embryo transfer (FET) cycles. METHODS Embryos were cryopreserved at the cleavage stage. On the d...

Objective To analyse factors influencing the outcome of frozen-thawed embryo transfer (FET) Method A retrospective analysis was performed in our center on 129 thawing cycles from March 2001 to April 2003. The related parameters were compared between conceived and non-conceived cycles. Results There were totally 129 clinical pregnancies in these transfers (pregnancy rate: 27.1%). Frozen-thawed e...

The methodologies used for cytometric sorting of fresh spermatozoa never allowed a clear resolution of sexual chromosomes of frozen-thawed semen. To devise a novel method for the production of bovine predefined sexed embryos using frozen-thawed semen, sorting efficiency of different protocols was studied using a new quantitative real-time PCR method to verify the purity of sexed semen. To this ...

The effect of modified vitrification was assessed on cellular development capability in mouse embryos cultured in vitro. In this study, 466 embryos (from zygote to morula stages) were vitrified then thawed embryos have been incubated for in vitro farther development up to blastocyst stage. Also, vitrification and thawing procedures were the same for all experimental groups. Mouse different embr...

A total of 41 patients requested thawing of supernumerary embryos in an intracytoplasmic sperm injection (ICSI) programme. Mean patient age was 30.8 +/- 3.8 years. Embryo freezing by the ultrarapid method was performed at room temperature in 3 mol/l DMSO and 0.25 mol/l sucrose. Total freezing time was 2.5 min including filling of the straw. In the thawing process, the embryos were removed from ...

Our purpose is to explore whether anti-dsDNA antibody, which was demonstrated to enter living cells and induced apoptosis, could adversely affect reproductive outcomes. A total of 259 women receiving the in vitro fertilization-embryo transfer (IVF) cycle were enrolled in this study, including 52 women with positive ANA and anti-dsDNA (ANA+/anti-dsDNA+ group), 86 women with positive ANA and nega...

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

Several laboratories have reported the birthofyoung from frozen-thawed mouse embryos transferred to recipient females (Whittingham, Leibo & Mazur, 1972; Wilmut, 1972) and frozen mouse embryos have been successfully transported from North America to the United Kingdom (Whittingham & Whitten, 1974). Of the domestic species, continued development in vivo and in vitro has been achieved with cattle ...

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...