OBJECTIVE To investigate the effects of desiccation (without cooling) and of oversaturation of the pneumoperitoneum on adhesion formation. DESIGN Prospective randomized trial. SETTING Academic research center. ANIMAL(S) BALB/c and NMRI female mice. INTERVENTION(S) The effect of desiccation using nonhumidified CO(2) on adhesion formation was evaluated in a laparoscopic mouse model. Body ...

PURPOSE To investigate the effect of desiccation on secretion of inflammatory cytokines in corneal epithelial cells and in the rat desiccation model. METHODS A human corneal epithelial cell line (CEPI) was grown in keratinocyte growth medium 2 (KGM2) to approximately 80% confluence. The medium was aspirated and dishes were left for 0 to 30 min with the cover left open to dry the cells (short-...

Some nematodes can survive almost complete desiccation by entering an ametabolic state called anhydrobiosis requiring the accumulation of protective molecules such as trehalose and LEA proteins. However, it is not known how anhydrobiotic organisms sense and regulate the response to water loss. Mitogen-activated protein kinases (MAPKs) are highly conserved signalling proteins that regulate adapt...

This review will focus on the acquisition of desiccation tolerance in the resurrection plant Craterostigma plantagineum. Molecular aspects of desiccation tolerance in this plant will be compared with the response of non-tolerant plants to dehydration. Unique features of C. plantagineum are described like the CDT-1 (Craterostigma desiccation tolerance gene-1) gene and the carbohydrate metabolism...

Salmonella spp., one of the most common foodborne bacterial pathogens, has the ability to survive under desiccation conditions in foods and food processing facilities for years. This raises the concerns of Salmonella infection in humans associated with low water activity foods. Salmonella responds to desiccation stress via complex pathways involving immediate physiological actions as well as co...

BACKGROUND Diverse organisms across taxa are desiccation tolerant, capable of surviving extreme water loss. Remarkably, desiccation tolerant organisms can survive years without water. However, the molecular mechanisms underlying this rare trait are poorly understood. RESULTS Here, using Saccharomyces cerevisiae, we show that intracellular trehalose is essential for survival to long-term desic...

Overwintering insects may experience extreme cold and desiccation stress. Both freezing and desiccation require cells to tolerate osmotic challenge as solutes become concentrated in the hemolymph. Not surprisingly, physiological responses to low temperature and desiccation share common features and may confer cross-tolerance against these stresses. Freeze-tolerant larvae of the goldenrod gall f...

BACKGROUND AND AIMS Active growth in post-embryonic sporophytes of desert mosses is restricted to the cooler, wetter months. However, most desert mosses have perennial gametophytes. It is hypothesized that these life history patterns are due, in part, to a reduced desiccation tolerance for sporophytes relative to gametophytes. METHODS Gametophytes with attached post-embryonic sporophytes of T...

We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the senescence-related gene smp-30. Desat2 decreased during recovery from desicca...

Nematodes are the dominant soil animals of the Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however, little is known about gene regulation by Antarctic nematodes that can survive multiple types and incidences of environmental stress. In order to reveal th...