Using DNA probes specific for the three members of the bean (Phaseolus vulgaris L.) phenylalanine ammonia-lyase (PAL) gene family, we have studied the effects of the product of the PAL reaction, trans-cinnamic acid (CA), on the appearance of individual PAL transcripts in suspension cultured bean cells. Concentrations of CA in excess of 10(-4) molar inhibited appearance of elicitor-induced trans...

Phytoalexin biosynthesis is part of the defense mechanism of soybean (Glycine max) plants against attack by the fungus Diaporthe phaseolorum f. sp. meridionalis (Dpm), the causal agent of stem canker disease. The treatment of soybean cotyledons with Dpm elicitor or with sodium nitroprusside (SNP), a nitric oxide (NO) donor, resulted in a high accumulation of phytoalexins. This response did not ...

Addition of an elicitor preparation from Verticillium dahliae to soybean or cotton cell suspension cultures induces the formation of the phytoalexins, glycelollin or sesquiterpene aldehydes, respectively. Recent work (PS Low, PF Heinstein 1986 Arch Biochem Biophys 249: 472-479) has shown that the induction of phytoalexin biosynthesis in these cells is preceded by rapid changes in the plant cell...

Protoplasts, Soybean Cell Suspension Cultures, Phytoalexin Production, Phytophthora megasperm a f. sp. glycinea, Glucan Elicitor Protoplasts isolated enzymatically from suspension-cultured cells of soybean (Glycine max L. Merr., cv. Harosoy 63) were used to study the production of the isoflavonoid-derived phytoale­ xin, glyceollin. A large enhancement in the in vivo rates of synthesis and catal...

B. Moerschbacher, B. Heck, K. H. Kogel, O. Obst, and H. J. Reisener Institut für Biologie III der RWTH Aachen, Worringer Weg, D-5100 Aachen, Bundesrepublik Deutschland Z. Naturforsch. 41c, 839—844 (1986); received April 4/June 9, 1986 Triticum aestivum, Puccinia graminis f. sp. tritici, Hypersensitive Response, Lignification, Elicitor The genuine biotic elicitor from germ tube walls of Puccinia...

background: sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of taxol. objective: to increase taxol production the cdna encoding 3-hydroxy-3-methylglutaryl-coa reductase (hmgr) from iranian hazel (genebank accession numb...

in this study the effect of different concentrations of some abiotic elicitors (salicylic acid, jasmonic acid, cadmium chloride and sodium chloride) with different incubation times (3, 6, 9 and 12) on azadiractin production and cell growth in the cell culture of the iranian native neem were investigated. cell growth rate was measured by changes in dry cell weight and it was used as a basis for ...

The elicitor-induced incorporation of phenylpropanoid derivatives into the cell wall and the secretion of soluble coumarin derivatives (phytoalexins) by parsley (Petroselinum crispum L.) suspension cultures can be potentiated by pretreatment of the cultures with 2,6-dichloroisonicotinic acid or derivatives of salicylic acid. To investigate this phenomenon further, the cell walls and an extracel...

Endopolygalacturonase isolated from culture ifitrates of the fungus Rhizopus stolonifer was shown previously to act as an elicitor ofbiosynthetic capacity for the antifungal agent, casbene, in castor bean (Ricinus commais L.) seedlings (S.-C. Lee, C.A. West 1961 Plant Physiology 67:633-639). Selective amidation of exposed carboxyl groups of the pure fungal endopolygalacturonase using intermedia...

sulforaphane (sfn) is an isothiocyanate which is produced through glucoraphanin hydrolysis via myrosinase activity. in the present study, sfn content and peroxidase activity has been assessed in treated lepidium draba seedlings with different concentrations of iron and copper during 8 and 16 hours treatment. the results showed that the sfn content drastically elevated at treatment with 1 mg/l f...