نتایج جستجو برای: electrophoresis
تعداد نتایج: 50636 فیلتر نتایج به سال:
A novel electrophoretic separation technique is presented, where continuous demonstrated using free flowing liquid foams. Continuous foam electrophoresis combines the principle of capillary and interactions between analytes electrical double layer, with ability Free Flow Electrophoresis to continuously separate recover automatically. used provide a network deformable micro nano channels high su...
isolation of cell free fetal dna (cffdna) from maternal serum usually leads to very low concentrations of dna impeding further resolving through conventional methods of electrophoresis. although several protocols have been described for capillary electrophoresis (ce) of double stranded dna, they usually need using special polymers or coated capillaries which degrade over time. herein, we propos...
Here we set forth the first from a series of reports devoted to history capillary electrophoresis. In this opening part, go more than two centuries back in time and revisit original discoveries electrolysis, electrophoresis electroosmosis. We emphasize essential role brilliant invention 1799 by Alessandro Volta, Voltaic pile, basically battery delivering constant-flow electricity, which has mad...
we have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-de). this modified version of 2-de is not only simpler and easier but also faster than all the currently available methods. in this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-...
background : glioblastoma multiforme (gbm) or grade iv astrocytoma is the most common and lethal adult malignant brain tumor. several of the molecular alterations detected in gliomas may have diagnostic and/or prognostic implications, proteomics has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses. method: we extracted protein...
alpha-amylase e.c.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as pseudomonas and asperigllus, as well as muscles and ovarian tubes.1.2 alpha-amylase from bacillus subtilis #1024(a tcc 465)* was purified with a highest degree of purity in our laboratory (31.59 u/mg). the extracellular alpha-amylase was subjected to different pu...
multiple myeloma (mm) characterized by proliferation of plasma cells in bone marrow and production of monoclonal immunoglobulin's. recently, arsenic trioxide (ato), has been considered for treatment refractory mm. we assessed the safety and efficacy of ato for patients with refractory mm. a phase 2, study of arsenic trioxide was conducted in 12 mm patients, whose refractory to two standard ther...
a capillary electrophoresis method was used for assay of some degradation products of carvedilol. the optimized parameters were as; running buffer 80 mm acetate dissolved in methanol/ethanol mixture (65:35 v/v), applied voltage of 19 kv, temperature is 20 ºc and the wavelength range of 200-350 nm. the results indicate that the proposed ce method could effectively separate carvedilol from its de...
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