نتایج جستجو برای: electrochemical immunosensor

تعداد نتایج: 52102  

Journal: :Frontiers in bioscience : a journal and virtual library 2005
C M Li Wei Chen Xu Yang C Q Sun Crystal Gao Z X Zhao Jaymie Sawyer

A simple and sensitive electrochemical immunosensor with impedance labelless detection and novel data processing method was investigated. One-step copolymerization was used to electrochemically deposit an antibody impregnated polypyrrole film on a glassy carbon electrode surface for the immunosensor. Impedance measurements provided a labelless or reporterless method to detect antibody (Ab)-anti...

2014
Wen Ping Zhao Guo Zhao Dong Fei Chen Zhi Huai Mao Xiang You Wang

Compared with conventional methods, electrochemical immunosensors have many advantages, such as low cost, high sensitivity, and rapid detection, and has certain prospects for realizing real-time-monitoring. In this paper, a design of portable pesticide residues detection instrument was presented based on an electrochemical impedance immunosensor. Firstly, we studied on an impedance immunosensor...

Journal: :The Analyst 2013
Marta M P S Neves María Begoña González-García Henri P A Nouws Agustín Costa-García

The first electrochemical immunosensor (EI) for the detection of antibodies against deamidated gliadin peptides (DGP) is described here. A disposable nanohybrid screen-printed carbon electrode modified with DGP was employed as the transducer's sensing surface. Real serum samples were successfully assayed and the results were corroborated with an ELISA kit. The presented EI is a promising analyt...

2016
Francesca Malvano Donatella Albanese Alessio Crescitelli Roberto Pilloton Emanuela Esposito

An impedimetric label-free immunosensor on disposable screen-printed carbon electrodes (SPCE) for quantitative determination of Ochratoxin A (OTA) has been developed. After modification of the SPCE surface with gold nanoparticles (AuNPs), the anti-OTA was immobilized on the working electrode through a cysteamine layer. After each coating step, the modified surfaces were characterized by cyclic ...

2017
Sivaranjani Devarakonda Renu Singh Jyoti Bhardwaj Jaesung Jang

Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanopartic...

Journal: :Chemical communications 2015
Hua Xie Qiqi Zhang Qin Wang Yaqin Chai Yali Yuan Ruo Yuan

In this work, we proposed a novel electrochemical immunosensor for sensitive detection of the total protein of Nosema bombycis based on Fe3O4 nanoparticles (Fe3O4NPs) as catalyst to electrocatalyze the reduction of methylene blue (MB) with the aid of Fe3O4NPs-DNA dendrimers for the signal amplification.

Journal: :The Analyst 2011
Yafeng Wu Songqin Liu Lin He

We present a novel immunosensor by using polymerization-assisted signal amplification strategy coupled with electrochemical detection. A sandwich immunoassay process was used to immobilize a polymerization reaction center, the initiator-conjugated polyclonal prostate specific antigen (PSA) or polyclonal carcinoembryonic antigen (CEA) antibodies on the surface of the electrode. Activator generat...

Journal: :Talanta 2014
Yanqiu Ge Jie Wu Huangxian Ju Shuo Wu

An ultrasensitive enzyme-free electrochemical immunoassay was developed for detection of the fg/mL level carcinoembryonic antigen (CEA) by using a double strand DNA@Au nanoparticle (dsDNA@AuNP) tag and hexaammineruthenium(III) chloride (RuHex) as the electroactive indicator. The dsDNA@AuNP was synthesized by one-pot hybrid polymerization of dsDNA on initiator DNA modified AuNPs via hybridizatio...

Journal: :Biomaterials 2009
Ying Zhuo Pei-Xi Yuan Ruo Yuan Ya-Qin Chai Cheng-Lin Hong

The preparation, characterization and application of a three-layer magnetic nanoparticle composed of an Fe(3)O(4) magnetic core, a Prussian Blue (PB) interlayer and a gold shell (it can be abbreviated as Au-PB-Fe(3)O(4)) for an ultrasensitive and reproducible electrochemical immunosensing fabrication were described for the first time in this work. With the employment of the Au-PB-Fe(3)O(4) nano...

2010
Michal Wasowicz Malgorzata Milner Dorota Radecka Krystyna Grzelak Hanna Radecka

Immunosensors based on gold electrodes (electrochemical) or gold discs (optical) modified with 1,6-hexanedithiol, gold nanorods and Anti-His (C-term) monoclonal antibody F(ab') fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhibitor (rSPI2-His(6)). Electrochemical impedance spectroscopy (EIS) and surface plasmon resonance ...

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