An interference-free amperometric biosensor for the quantitative xanthine analysis is described. Xanthine was monitored through the electrochemical reduction of hydrogen peroxide (produced upon its enzyme-catalyzed oxidation) at a constant applied potential of -50 mV (vs. Ag/AgCl). The biosensor was designed on the basis of a graphite electrode modified with microquantities of platinum and pall...

Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an i...

A proximity-dependent surface hybridization strategy is employed for designing a "signal-on" electrochemical DNAzyme biosensor. By taking advantage of the high sensitivity of the PDSH strategy, and by realizing the enzymatic hydrolysis reaction in a homogenous system with a unimolecular design, the proposed biosensor shows a very high sensitivity to target molecules.

Recent trends and challenges in the electrochemical methods for the detection of DNA hybridization are reviewed. Electrochemistry has superior properties over the other existing measurement systems, because electrochemical biosensors can provide rapid, simple and low-cost on-field detection. Electrochemical measurement protocols are also suitable for mass fabrication of miniaturized devices. El...

In this paper a novel elctrochemical HbA1c biosensor based on flow injection and screen-printed electrode was developed. The reticulated vitreous carbon (RVC) electrode was modified with 3aminophenylboronic acid, chitosan (CS), and tetraenthoxyl silica (TEOS), which entrapped carbon nanotubes to form a sol-gel film at the surface of the screen-printed electrode. The electrochemical behavior of ...

A novel catechol (CA) biosensor was developed by embedding tyrosinase (Tyr) onto in situ electrochemical reduction graphene (EGR) on choline-functionalized gold nanoparticle (AuNPs-Ch) film. The results of UV-Vis spectra indicated that Tyr retained its original structure in the film, and an electrochemical investigation of the biosensor showed a pair of well-defined, quasi-reversible redox peak...

An electrochemical biosensor for kinase-catalyzed reactions is coupled with the thiophosphorylation of the substrate peptide using adenosine 5'-[gamma-thio] triphosphate (ATP-S) as the co-substrate.

We demonstrate a novel high-performance DNA hybridization biosensor with a carbon nanotubes (CNTs)-based nanocomposite membrane as the enhanced sensing platform. The platform was constructed by homogenously distributing ordered FePt nanoparticles (NPs) onto the CNTs matrix. The surface structure and electrochemical performance of the FePt/CNTs nanocomposite membrane were systematically investig...

A simple, selective and sensitive electrochemical biosensor has been developed to detect protease biomarker from Bacillus licheniformis, a recognized model of the biochemical warfare agent Bacillus anthracis. In this assay, the biosensor is constructed using a d-amine acid containing substrate peptide via self-assembly of cysteine residual at the C-terminal. A biotin modifier is labelled at the...

A tetragonal pyramid-shaped porous ZnO (TPSP-ZnO) nanostructure is used for the immobilization, direct electrochemistry and biosensing of proteins. The prepared ZnO has a large surface area and good biocompatibility. Using glucose oxidase (GOD) as a model, this shaped ZnO is tested for immobilization of proteins and the construction of electrochemical biosensors with good electrochemical perfor...