During 2002-2003, exotic Newcastle disease (END) virus caused a major outbreak among commercial and backyard poultry in southern California and adjacent states. The outbreak raised concerns regarding the protective immunity of commercially available vaccines for prevention and control of this virus in poultry. We sought to determine if existing commercial live and inactivated Newcastle disease ...

Avian influenza (AI) is a highly contagious disease causing significant economic losses worldwide. The aim of this study is to evaluate the effect of mannan-oligosaccharide (MOS) on tracheal and cloacal virus shedding in AI challenged broilers and contamination of environment with H9N2. A total of 300 1-day-old-broiler chicks were randomly divided into 3 groups (A, B and C) and supplemented 0.2...

In this study, the pathogenicity of A/Ch/It/5093/1999 H7N1 which had been isolated from chicken during the outbreak in Italy was assessed in chicken by experimental infection virus. Ten SPF chickens of four week-old were inoculated with this virus, and five chickens were inoculated with uninfected allantoic fluid. For determination of virus shedding, oropharyngeal and cloacal swabs were taken f...

This study is aimed at evaluating antibody responses, viral loads, clearance and growth rate of Tanzanian free-range local chicken (FRLC) challenged with LaSota strain Newcastle disease virus (NDV) as indicator traits for selection chickens breeding enhanced resistance to the economic value. Three popular ecotypes: Kuchi, Ching’wekwe Morogoro-medium from three ecological zones Tanzania we...

Vaccination against Newcastle disease (ND), a devastating viral of chickens, is often hampered by thermal inactivation the live vaccines, in particular tropical and hot climate conditions. In past, “thermostable” vaccine strains (I-2) were proposed to overcome this problem but previous comparative studies did not include formulation-specific factors commercial vaccines. current study, we aimed ...

Abstract Metagenomic next-generation sequencing (mNGS) holds promise as a diagnostic tool for unbiased pathogen identification and precision medicine. However, its medical utility depends largely on assay simplicity reproducibility. In the current study, we aimed to develop streamlined Illumina Oxford Nanopore-based DNA/RNA library preparation protocol rapid data analysis pipeline. The sequenci...

during 2004-2006, a total number of 22 tissue samples obtained from poultry flocks suspected to respiratory diseases submitted to avian virology laboratory of faculty of veterinary medicine, were prepared for avian influenza virus (aiv) isolation in embryonated chicken eggs, according to the standard method. rt-pcr was established on tissue samples using specific primers for h9 gene. amplified ...

Aseel and Naked neck are major chicken breeds of the tropics well-known for their thermotolerance robustness. However, both them especially very susceptible to Avian Influenza (AI) which cause huge mortalities. The role cytokines in relation pathology severity disease caused by endemic strain (H9N2) AIV these remained thus far unclear. aim this study was investigate effects H9N2 on expression l...

Traditionally, substrates for production of viral poultry vaccines have been embryonated eggs or adherent primary cell cultures. The difficulties and cost involved in scaling up these substrates in cases of increased demand have been a limitation for vaccine production. Here, we assess the ability of a newly developed chicken-induced pluripotent cell line, BA3, to support replication and growth...