A ratiometric fluorescent nanoprobe based on terbium functionalized carbon dots (CDs) was designed to detect dipicolinic acid (DPA) as an anthrax biomarker with high selectivity and sensitivity. CDs were generated by one-step synthesis using an ethylenediaminetetraacetic acid precursor, and served as a scaffold for coordination with Tb(3+) and a fluorescence reference.

Formation of thrombin during incubation of purified bovine prothrombin with purified staphylococcal metalloprotease has been investigated. Thrombin activity was estimated by examination of clotting time and by digestion of a synthetic substrate, Chromozym TH. The metalloprotease caused direct activation of prothrombin which was inhibited by the addition of ethylenediaminetetraacetic acid. Metal...

We read the report “Peculiar Cold-Induced Leukoagglutination in Mycoplasma pneumoniae Pneumonia” with great interest [1]. Kubota et al. [1] reported an interesting patient with Mycoplasma pneumoniae pneumonia who had leukoagglutination. They noted that this is a rare condition. We agree that the patient had leukoagglutination and Mycoplasma pneumoniae pneumonia. Nevertheless, the leukoagglutina...

Liposomes were incubated with an extract of human plantar stratum corneum. The liposomal lipids were hydrolysed, if composed of soy-bean phosphatidylcholine or phosphatidylglycerol. Rigid lipids were not degraded. The temperature optimum of the hydrolysis was between 30-35degC. CaCl enhanced, while EDTA reduced the rate of hydrolysis, indicating that the hydrolysis is due to a phospholipase A(2).

Liposomes were incubated with an extract of human plantar stratum corneum. The liposomal lipids were hydrolysed, if composed of soy-bean phosphatidylcholine or phosphatidylglycerol. Rigid lipids were not degraded. The temperature optimum of the hydrolysis was between 30-35°C. CaCl2 enhanced, while EDTA reduced the rate of hydrolysis, indicating that the hydrolysis is due to a phospholipase A2.

Table 1. Precision of Lactate and Pyruvatea (1970). Lactate method Pyi’uv.ts method 10. Davidson, D. F., Absorbance accuracy Mean, 50 Mean SO check on the LKB 8600 reaction rate anammol/tftsr CV, % n imol/IItsr CV, % 11 lyzer. J. Clin. Pathol. 29,758(1976). Within-batch precision ii. Bucher, T., Czak, R., Lamprecht, W., and 1.80 0.09 4.9 14 104 4.3 4.1 15 Latzko,E., In Methods of Enzymatic Anal-

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Transport behaviour of Lu(III) across a polypropylene hollow fibre-supported liquid membrane containing di(2-ethylhexyl)phosphoric acid (DEHPA) in dihexyl ether as a carrier has been studied. The donor phase was LuCl(3) in the buffer solution consisting of 0.2 M sodium acetate at pH 2.5-5.0. A miniaturised system with a single hollow fibre has been operated in a batch mode. The concentration of...

BACKGROUND Zinc (Zn) biofortification through foliar Zn application is an attractive strategy to reduce human Zn deficiency. However, little is known about the biofortification efficiency and bioavailability of rice grain from different forms of foliar Zn fertilizers. METHODOLOGY/PRINCIPAL FINDINGS Four different Zn forms were applied as a foliar treatment among three rice cultivars under fie...

A new method for determining the glomerular filtration rate was analysed prospectively. The method uses an x ray fluorescence technique to measure disappearance from the plasma of injected non-ionic iodinated contrast media. Eighty seven patients were studied. Fifty four had an intravenous dose of 100 ml iohexol (Omnipaque) and 33 had 50 ml iohexol. Clearances ofchromium-51 labelled edetic acid...