2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of purple-grained Tibetan hulless barley variety Nerumuzha and white-grained Kunlun 10. The HvnHID gene includes 981 bp open reading frame encodes a protein 327 amino acids. It has typical Abhydrolase_3 domain (78-306) belongs to carboxylesterase (CXE) famil...