We present a technique which combines an in vitro tracer injection protocol, which uses a series of electrical and pressure pulses to increase dye uptake through electroporation in brain explants with targeted laser illumination and matching filter goggles during the procedure. The described technique of in vitro electroporation by itself yields relatively good visual control for targetting cer...

The Coruh Valley, located in Northeastern Turkey, is one of the most important centers of diversity in pomegranate in Turkey. In this study, we attempted to characterize 19 promising pomegranate genotypes originating from the Coruh Valley in using fluorescent dye AFLP markers and capillary electrophoresis. Four AFLP primer combinations were used, generating a total of 297 fragments, 213 of whic...

Characterization of natural allelic diversity and understanding the genetic structure and linkage disequilibrium (LD) pattern in wild germplasm accessions by large-scale genotyping of informative microsatellite and single nucleotide polymorphism (SNP) markers is requisite to facilitate chickpea genetic improvement. Large-scale validation and high-throughput genotyping of genome-wide physically ...

Single particle characterization has become increasingly relevant for research into extracellular vesicles, progressing from bulk analysis techniques and first-generation to comprehensive multi-parameter measurements such as nano-flow cytometry (nFCM). nFCM is a form of flow that utilizes instrumentation specifically designed nano-particle analysis, allowing thousands EVs be characterized per m...

Abstract Ultrahigh-plex single-cell spatial phenotyping is revolutionizing immuno-oncology research. To enable in-depth characterization of the tumor microenvironment (TME), we have developed and validated pre-optimized antibody panels on cancer tissues to reveal key cell types cellular architecture using PhenoCycler™-Fusion system. We designed multiple that answer specific biological questions...

low-cost grapefruit peel (agricultural waste) was used for removal of reactive blue19 dye fromaqueous solutions. the process was studied as a function of contact time, initial dye concentration andph. adsorption process was attained to the equilibrium within 45 min for initial dye concentrations of 50,75, and 100 mg l-1. an acidic medium was the optimum condition for adsorption of dye at roomte...

A novel class of nonionic, carbohydrate-based surfactants has been synthesized from the plant polysaccharide xyloglucan. Enzymatic hydrolysis of xyloglucan yielded a series of well-defined, highly branched oligosaccharides that, following reductive amination, were readily conjugated with fatty acids bearing C8 to C18 chains under mild conditions. The critical micelle concentration, determined b...

introduction:thepowderedactivatedcarbontreatment(pact)hasbeenproposedasanalternativeinwastewatertreatmentandwastewaterbiomassprotectionagainsttoxicsubstances.thisstudyaimstoevaluatepactperformanceintreatingdyes,acidorange,andremazolbrilliantreactiveblue. methods:thisempirical-appliedresearchwascarriedoutinpilotscaleinwhichdifferentdyeremoval systems were tested:activatedsludge,pac,andthecombine...