Vascular leakage in the brain is a major complication associated with brain injuries and certain pathological conditions due to disruption of the blood-brain barrier (BBB). We have developed an optical imaging method, based on excitation and emission spectra of Evans Blue dye, that is >1000-fold more sensitive than conventional ultraviolet spectrophotometry. We used a rat thromboembolic stroke ...

The purpose of this in vitro study was to evaluate the effect of neodymium:yttrium-aluminum-garnet (Nd:YAG) laser irradiation on intracanal dentin surface by SEM analysis and its interference in the apical seal of filled canals. After endodontic treatment procedures, 34 maxillary human incisors were randomly assigned to 2 groups. In the negative control group (n=17), no additional treatment was...

The magainins, peptide antibiotics secreted by the frog Xenopus laevis, have previously been shown to permeabilize phospholipid vesicles. To elucidate the mechanism of permeabilization, we have conducted detailed kinetic studies of magainin 2 amide (mgn2a)-induced release of 6-carboxyfluorescein from vesicles of phosphatidylserine. The results show that dye release occurs in (at least) two stag...

introduction: in apical surgery, after apicoectomy and retro-preparation of canal, a retrofil material is applied to seal the apical region of the root canal. mineral trioxide aggregate (mta) is the gold standard material for this purpose. changing water/powder ratios of mta affects its properties. the purpose of this in vitro study was to determine the effect of liquid/powder ratio of retrofil...

AIM The aim of this study was to assess the apical sealing ability of Resilon obturation material. METHODS AND MATERIALS Forty-two single rooted extracted human teeth were selected and randomly divided into three groups. They were obturated using lateral condensation with gutta-percha and AH 26/AH plus and Resilon; also, 12 root canals were used as control groups. The apical leakage was measu...

objective: one of the principle purposes of root canal obturation is to obtain hermetic sealing of the root canal system. according to the development of technology, many materials are now used in root canal filling. an in vitro dye leakage study was performed to evaluate the apical sealability of white-colored mineral trioxide aggregate (wmta) and gray-colored portland cement (gpc) when used a...

We studied pial vessels in vivo in mice, examining under mercury light the permeability of these vessels to sodium fluorescein. Topical application of hypertonic solutions of NaCl caused leakage of fluorescein. However, putative chemical mediators of leakage, such as histamine, serotonin, arachidonic acid, and bradykinin, all failed to increase permeability to the dye. Apparent increases in per...