A unique interaction between the cyanine dye and negatively charged quantum dot is used to construct a signal-on biaptameric quantum dot (QD) Förster resonance energy transfer (FRET) beacon for protein detection and distinct aptamer characterization. The beacon comprises a pair of aptamers, one intercalated with the cyanine dye (YOYO-3) and the other conjugated to a negatively charged, carboxyl...

We studied the spontaneously active in vitro tadpole brainstem and recorded whole nerve respiratory activity while simultaneously visualizing intracellular pH (pHi) dynamics using the pH-sensitive dye, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester (BCECF, AM). The isolated, superfused tadpole brainstem is well oxygenated and retains synaptic connectivity among res...

We demonstrate the fabrication and characterization of a novel, inexpensive microchip capable of laser induced fluorescence (LIF) detection using integrated waveguides with built-in optical filters. Integrated wavelength-selective optical waveguides are fabricated by doping poly(dimethysiloxane) (PDMS) with dye molecules. Liquid-core waveguides are created within dye-doped PDMS microfluidic chi...

School of Chemistry, ARC Centre of Excelle Technology, University of New South Wa E-mail: [email protected] Australian Centre for NanoMedicine, Univ Wales 2052, Australia EMBL Australia Node in Single Molecule Advanced Molecular Imaging, University o 2052, Australia † Electronic supplementary information ( simultaneous setup and mathematical potential dye cytotoxicity, selecting the el...

Enhancement in the performance of Silicon solar cell can be achieved via luminescent down-shifting (LDS) of the incident light. Lumogen Violet dye and Tb(ant)3.2H2O complex were characterized to determine their suitability for inclusion in LDS layers. LDS layer characterization techniques are discussed and preliminary results from PV cell/LDS layer devices are presented.

Angle-resolved reflection and emission spectra of metal gratings consisting of subwavelength grooves and immersed into rhodamine B and rhodamine 19 solutions are presented. The measured reflection and emission dispersion diagrams reveal the surface plasmon polaritons positions and strong plasmon-mediated emission enhancement, respectively. The same grating could be easily reused for the charact...

The synthesis and characterization of a highly photostable bromo-substituted BODIPY dye (I) fused-ring-expanded with thienopyrrole moieties is reported. The results of MTT assays and flow cytometric analyses in living HeLa cells demonstrate that I has a high singlet oxygen quantum yield (ΦΔ = 0.63) and exhibits photocytotoxicity upon irradiation in the NIR region making it potentially suitable ...

We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluo...