نتایج جستجو برای: dutp

تعداد نتایج: 3480  

Journal: :Nucleosides, nucleotides & nucleic acids 2011
Gábor Merényi Júlia Kovári Judit Tóth Enik Takács Imre Zagyva Anna Erdei Beáta G Vértessy

dUTPase is involved in preserving DNA integrity in cells. We report an efficient dUTPase silencing by RNAi-based system in stable human cell line. Repression of dUTPase induced specific expression level increments for thymidylate kinase and thymidine kinase, and also an increased sensitization to 5-fluoro-2'-deoxyuridine and 5-fluoro-uracil. The catalytic mechanism of dUTPase was investigated f...

Journal: :Nucleosides, nucleotides & nucleic acids 2006
Christopher Lacenere Meil K Garg Brian M Stoltz Stephen R Quake

The ability of eight commercially available thermophilic DNA polymerases to sequentially incorporate fluorescently labeled nucleotides sequentially was analyzed by a gel based primer extension assay. Cy5-dUTP or a variant nucleotide in which the linker had been lengthened by 14 atoms between the dye and the nucleobase were compared. We found that the Cy5-dUTP with a longer linker resulted in lo...

Journal: :Trakya university journal of natural sciences 2021

SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily. Distinctive elements (ENV GAG) distributions in barley genome were observed by using fluorescent situ hybridization (FISH). We performed PCR obtain tetramethylrhodamine-dUTP (TRITC)-labelled probes. Localizations of ENV GAG domains demonstrated under c...

Journal: :Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences 2013

Journal: :Anesthesiology 2014
Wen-Hsien Lu Kai-Sheng Hsieh Pei-Jung Lu Yi-Shan Wu Wen-Yu Ho Pei-Wen Cheng Chi-Cheng Lai Michael Hsiao Ching-Jiunn Tseng

BACKGROUND Bilateral lesions of nucleus tractus solitarii in rat result in acute hypertension, pulmonary edema, and death within hours. The hypertension results from excessive catecholamine release. Catecholamine can activate connexin43 to regulate cell death. There is no study investigating the cardiopulmonary impacts of different adrenergic blockers and apoptosis mechanism in rat model. MET...

Journal: :Stroke 2012
Xiaoxia Zhu Luyang Tao Emiri Tejima-Mandeville Jianhua Qiu Juyeon Park Kent Garber Maria Ericsson Eng H Lo Michael J Whalen

BACKGROUND AND PURPOSE Traumatic and ischemic brain injury induce plasmalemma permeability and necrosis; however, no studies have examined these aspects of cellular injury in intracerebral hemorrhage models. METHODS In vivo propidium iodide (PI) and YOYO-1 were used to assess plasmalemma damage after collagenase-induced intracerebral hemorrhage in mice. Ex vivo aspartylglutamylvalylaspartic a...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید