Genome-wide experimental studies in Saccharomyces cerevisiae reveal that autonomous replicating sequence (ARS) requires an essential consensus sequence (ACS) for replication activity. Computational studies identified thousands of ACS like patterns in the genome. However, only a few hundreds of these sites act as replicating sites and the rest are considered as dormant or evolving sites. In a bi...

This report describes a distributed implementation of an interpreter for the computation orchestration language ORC. While ORC includes primitives for distributed computation in the form of “sites”, these have limitations and it is difficult to convert a non-distributed ORC program into a distributed one. I therefore introduce a new syntax for annotating distributable expressions in an existing...

BACKGROUND The limitation of carbonyl reduction represents one possible way to increase the effectiveness of anthracycline doxorubicin (DOX) in cancer cells and decrease its toxicity in normal cells. In vitro, isoquinoline derivative oracin (ORC) inhibited DOX reduction and increased the antiproliferative effect of DOX in MCF-7 breast cancer cells. Moreover, ORC significantly decreases DOX toxi...

The origin recognition complex (ORC), a heteromeric six-subunit protein, is a central component for eukaryotic DNA replication. The ORC binds to DNA at replication origin sites in an ATP-dependent manner and serves as a scaffold for the assembly of other key initiation factors. Sequence rules for ORC-DNA binding appear to vary widely. In budding yeast the ORC recognizes specific ori elements, h...

A bacterial strain designated Orc-4(T) was isolated from a freshwater spring in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain Orc-4(T) were facultatively anaerobic, Gram-reaction-negative, poly-β-hydroxybutyrate-accumulating, non-motile rods surrounded by a thick capsule and forming cream-white colonies. Growth occurred at 15-40 °C (optimum, 25-30 °C), at pH ...

The role of the natural HMR-E silencer in modulating replication initiation and silencing by the origin recognition complex (ORC) was examined. When natural HMR-E was the only silencer controlling HMR, the silencer's ORC-binding site (ACS) was dispensable for replication initiation but essential for silencing, indicating that a non-silencer chromosomal replicator(s) existed in close proximity t...

The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin....

An open resonance cell (ORC) with finite-length cylindrical mirrors is suggested for making absolute measurements of complex permittivity in the case of stretched cylindrical specimens or liquid dielectrics in cylindrical containers. For H-polarization, we report ORC features simulated in rigorous electromagnetic terms using a twodimensional (2-D) model of an open resonator with cylindrical mir...

The eukaryotic pre-replication complex is assembled at replication origins in a reaction called licensing. Licensing involves the interactions of a variety of proteins including the origin recognition complex (ORC), Cdc6 and the Mcm2-7 helicase, homologues of which are also found in archaea. The euryarchaeote Archaeoglobus fulgidus encodes two genes with homology to Orc/Cdc6 and a single Mcm ho...

Air-cooled condensers are widely used for Organic Rankine Cycle (ORC) power plants where cooling water is unavailable or too costly, but they are then vulnerable to changing ambient air temperatures especially in continental climates, where the air temperature difference between winter and summer can be over 40 C. A conventional ORC system using a single component working fluid has to be design...