Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin...

Changes in the physical properties (size, particle density, bulk density, shrinkage and porosity) and fluidization behaviour of cut green beans with change in moisture content during drying were investigated using a heat pump and fluidized bed dryers. Three length:diameter ratios 1:1, 2:1, 3:1 of cut green beans were considered. All drying experiments were conducted at 50 + 2 0 C and 13 + 2 % R...

The durability of adhesive joints is of special concern in structural applications and moisture has been identified as one of the major factors affecting joint durability. This is especially important in applications where joints are exposed to varying environmental conditions throughout their life. This paper presents a methodology to predict the stresses in adhesive joints under cyclic moistu...

Injecting supercritical CO2 (scCO2) into brine-filled fracture-dominated reservoirs causes brine displacement and possibly evaporite precipitations that alter the fracture space. Here, we report on isothermal laboratory experiments scCO2-induced flow-through drying in a naturally fractured granodiorite specimen under effective normal stresses of 5-10 MPa, where two regimes are identified. A nov...

Enzymes subjected to freeze-thawing are known to be protected by polymers that are preferentially excluded from the hydrated surface of proteins [reviewed in Carpenter et al. (1994) ACS Symp. Ser. 567, 134-147]. Preferentially excluded solutes are also known to stabilize quaternary structure, which enhances the thermostability of multimeric proteins in aqueous systems. Also, it has been suggest...

To demonstrate the adaptation of lactic acid bacteria to environmental stresses can improve cell viability during freeze-drying, Lactobacillus plantarum VAL6 strain was cultured under different stress conditions, including temperature, pH, and increase in CO2 concentration. The results densitometry analysis showed that able survive harsh conditions such as at pH 2.5, temperature 47oC, anaerobic...

Desiccation cracks in colloidal deposits occur to release the excess strain energy arising from competition between drying induced shrinkage of deposit and its adhesion substrate. Here we report remarkably different morphology desiccation dried patterns formed by evaporation sessile drops containing colloids on elastomer (soft) or glass (stiff) substrates. The change crack pattern, i.e., radial...

BACKGROUND Drought stress is one of the most important abiotic stresses and the main constraint to rice agriculture. MicroRNA-mediated post-transcriptional gene regulation is one of the ways to establish drought stress tolerance in plants. MiRNAs are 20-24-nt regulatory RNAs that play an important role in regulating plant gene expression upon exposure to biotic and abiotic stresses. METHODOLO...

Propionibacterium freudenreichii is a beneficial food-grade actinobacterium, widely implemented, and thus consumed, in various food products. As the main application, P. used as cheese-ripening starter, mostly hard type cheeses. Indeed, during manufacture of “Swiss-type” cheeses (or opened-body cheeses), technological process favors propionibacteria growth, well corresponding propionic fermenta...

to reduce the storing space and handling costs for hay/straw bales, recompressing the original bales after adequate hay drying is recommended. in this project, a recompression machine was designed, constructed and evaluated. solidwork and nastran software were employed to model, and to analyze the stresses in the components of the machine, respectively. the components of the machine consisted o...