It had previously been shown (l-3) that extracts of pigeon liver could convert pantetheine and ATP to CoA’ in low yield. It was found, however, that fresh extracts of pigeon liver, from which CoA has been removed with Dowex 1 rather than by aging, effected a much more vigorous synthesis of CoA. Since the aging procedure apparently inactivated the enzyme responsible for the conversion of panteth...

It has been found that certain urinary quinoline derivatives could be eluted from Dowex 50 (H+) columns by washing with a large volume of water (l-3). The present study concerns an examination of urine from normal swine and rabbits for various quinoline derivatives. Seven quinoline derivatives were identified in the urine of these two species, including two apparently new urinary products and t...

Nagstatin, a new inhibitor of N-acetyl-beta-D-glucosaminidase (NAG-ase) was discovered in the fermentation broth of Streptomyces amakusaensis MG846-fF3. It was purified by chromatography on Dowex 50W, Avicel and Sephadex LH-20 followed by the treatment of active carbon and then isolated as colorless powder. Nagstatin has the molecular formula of C12H17N3O6. It is competitive with the substrate,...

A rapid, accurate method is described for determining magnesium in red blood cells. Dowex 50 resin was used to separate magnesium from calcium and other cellular constituents. Magnesium in the eluate was titrated with EDTA, with Eriochrome Black T as indicator; the end point was determined spectrophotometrically. Magnesium in the erythrocytes of 17 healthy adults averaged 4.57 ± 0.16 (SD) mEq/l...

Mitochondria isolated from etiolated shoots of com ( Zen mays), wheat (Tddk aestum, baley (HM "*are), soybean ( Glychie max L. Merr.), and mung bean ( Phasebhis awueus) exhibited a prol-pndent 02 uptake subject to respiratory controL ADP/O ratios with proline as substrate were intermediate between ratios obtained with exogenous NADH and malate + pyruvate as substrates. Isotope studies showed pr...

Since the isolation of hydroxylysine as the picrate from the phosphotungstic acid-precipit>able fraction of gelatin acid hydrolysates by Van Slyke, Hiller, Dillon, and MacFadyen (I), other isolation procedures have been reported (2, 3). The most, recent is t.hat of Sheehan and Bolhofer (4) who used chromatographic: techniques in part to obtain analytically pure hydroxylysine from gelatin.’ The ...