نتایج جستجو برای: dmem

تعداد نتایج: 2025  

2015
Akio Takayanagi

INTRODUCTION We evaluated the potential preventive effects and mechanisms of intravenously preloaded mesenchymal stem cells (MSCs) for erectile dysfunction (ED) in a cavernous nerve (CN) injury model. METHODS Male Sprague-Dawley (SD) rats were used for this study. Rats were randomized into two groups. One group was intravenously preloaded with MSCs (1.0 × 10(6) cells in 1 mL total fluid volum...

2015
Chawalit Siriboon Yu-Hsuan Lin Michel Kere Chun-Da Chen Lih-Ren Chen Chien-Hong Chen Ching-Fu Tu Neng-Wen Lo Jyh-Cherng Ju

We attempted to isolate ES cell lines using inner cell masses from high-quality cloned porcine blastocysts. After being seeded onto feeders, embryos had better (P < 0.05) attachment, outgrowth formation and primary colonization in both 2× and 3× aggregated cloned embryos (62.8, 42.6 and 12.8% vs. 76.2, 55.2 and 26.2%, respectively) compared to the non-aggregated group (41.6, 23.4 and 3.9%). Eff...

1993
Stephen P. Seslar Toshikazu Nakamura Stephen W. Byers

Hepatocyte growth factor/scatter factor (HGF/SF) is a stromallv de rived modulator of epithelial cell proliferation and morphology. To better assess the potential role of HGF/SF in tumor progression we sought to identify factors and biological conditions which regulate its expression. We show that several adult human primary fibroblast cultures from breast and prostate produce HGF/SF. HGF expre...

Journal: :Cell transplantation 2015
Chao Chi Fei Wang Bo Xiang Jixian Deng Shangdian Liu Hung-Yu Lin Kanmani Natarajan Gang Li Lei Wang Jian Wang Francis Lin Darren H Freed Rakesh C Arora Hongyu Liu Ganghong Tian

Adipose-derived stem cells (ASCs) from subcutaneous and visceral adipose tissues have been studied individually. No studies have compared their abilities in treatment of heart failure. This study was designed to evaluate whether ASCs from the two sources could provide a long-term improvement of cardiac function in infarcted hearts. Rat subcutaneous and visceral adipose tissues were excised for ...

Journal: :American journal of translational research 2017
Jingling Su Chenxi Xie Yanyun Fan Weizi Cheng Yiqun Hu Qingwen Huang Huaxiu Shi Lin Wang Jianlin Ren

BACKGROUND This study aimed to investigate the influence of IL-25 on the capacity of mesenchymal stem cells (MSCs) to induce intestinal epithelial cell regeneration. METHODS The CD4+IL-25R+ cells and LGR5+IL-25R+ cells in colonic mucosa of Crohn's disease (CD) patients, ulcerative colitis (UC) patients and healthy controls were detected by immunofluorescence staining, and the CD4+IL-25R+ cell...

Journal: :Investigative ophthalmology & visual science 2006
Michael P Fautsch Cindy K Bahler Anne M Vrabel Kyle G Howell Nils Loewen Wulin L Teo Eric M Poeschla Douglas H Johnson

PURPOSE A previous study by the authors has shown that recombinant myocilin purified from a prokaryotic expression system increases outflow resistance in cultured human anterior segments. The present study was performed to determine whether full-length myocilin purified from a human trabecular meshwork cell expression system alters outflow resistance after infusion into human anterior segments....

Journal: :Jurnal Teknologi Laboratorium 2022

Cell culture requires a suitable medium and environment for in vivo conditions. Conditions are created by regulating temperature, pH, oxygen, CO2, osmotic pressure, surfaces to adhere cells, nutrients, vitamins, protection against toxic substances, hormones, growth factors that regulate cell differentiation. In general, good occurs at pH of 7.0 - 7.6. Fetal Bovine Serum (FBS) is one the supplem...

Journal: :Coatings 2023

Degradable and non-degradable biomaterials are two categories that can be used to classify the existing biomaterials, being a solution for eliminating second surgical intervention of implant when tissue has properly recovered. In present paper, effect deposition temperature on structure, morphology, hardness, electrochemical evaluation, degradation properties functional peptides adhesion Mg Si-...

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