Superoxide dismutase (EC-SOD) controls the level of superoxide in the extracellular space by catalyzing the dismutation of superoxide into hydrogen peroxide and molecular oxygen. In addition, the enzyme reacts with hydrogen peroxide in a peroxidase reaction which is known to disrupt enzymatic activity. Here, we show that the peroxidase reaction supports a site-specific bond cleavage. Analyses b...

Evidence was also obtained that the dismutation of acetaldehyde was accompanied by phosphorylation of ADP, presumably via intermediary acetyl phosphate, to form ATP. During the course of these studies, Hayward (4) isolated a clostridium from a medium that contained choline as a major source of energy. This organism was found to catalyze the same over-all fermentation of choline as that of the v...

An automated multi-pumping flow system was developed for the in-line generation of singlet oxygen ((1)O(2)) and subsequent assessment of the scavenging capacity against this reactive species. (1)O(2) was generated by dismutation of hydrogen peroxide catalyzed by molybdate ions. The evaluation of the scavenging capacity was based on the inhibition of the chemiluminescence reaction of luminol wit...

There are few ways to develop a high concentration of superoxide in solutions of physiological significance, and by far the most useful of these, pulsed radiolysis of oxygenated solutions [1 ], requires very specialized and expensive research equipment. One of the long term goals of this laboratory has been to develop alternative procedures for examining the chemistry of superoxide ion in aqueo...

THE literature on bacterial metabolism contains references which suggest that the anaerobic dismutation of pyruvic acid which we have shown to occur in animal tissues (see the preceding paper) may also take place in certain bacteria. Barron et al. [1932; 1933; 1934; 1936], for instance, described experiments concerning the oxidation of pyruvic acid in Gonococcus and showed that there is a speci...