introduction: in this study, using the phenotypic and genotypic methods, oxacillin susceptibility in staphylococcus aureus (s. aureus) strains isolated from patients at two government hospitals in ilam, iran was tested. materials and methods: out of 200 s. aureus isolates from different human clinical specimens consisting of blood (31%), wound (20%), urine (21%), catheters (7%), sputum (12%), o...

Twelve lots of commercially prepared Mueller-Hinton agar from four medium manufacturers were evaluated for performance with recommended quality control organisms, medium pH, agar depth and uniformity, and characteristics of the zones of inhibition. Only 2 of 12 lots were acceptable for disk diffusion susceptibility tests. Significant problems were observed with the preparation and pouring of th...

OBJECTIVE To determine, by phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus strains isolated from pediatric and neonatal intensive care unit patients seen at the University Hospital of the Botucatu School of Medicine. METHODS A total of 100 S. aureus strains isolated from the following materials were studied: 25 blood cultures, 21 secretions, 12 catheters, 3...

The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 microg) with oxacillin disks (1 microg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-n...

Due to the increase in multidrug-resistant Enterobacteriaceae, the interest in older antimicrobial agents, like fosfomycin, has increased. In this study, we used agar dilution for testing susceptibilities to fosfomycin in a collection of 107 carbapenem-nonsusceptible Enterobacteriaceae isolates, of which 80 produced various types of carbapenemases, including KPC, VIM, NDM, and OXA-48. Overall, ...

BACKGROUND AND AIMS Enterococci are Gram-positive cocci that often occur in pairs (diplococci) or short chains. Be-side developing high level of antibiotic resistance, these bacteria can cause wide range of disease in human, thus to help provide an effective treatment for infections caused by this genus, this study was conceived to provide information on Enterococcus faecalis Antibiotic resista...