Here we report a systematic approach for predicting subcellular localization (cytoplasm, mitochondrial, nuclear, and plasma membrane) of human proteins. First, support vector machine (SVM)-based modules for predicting subcellular localization using traditional amino acid and dipeptide (i 1) composition achieved overall accuracy of 76.6 and 77.8%, respectively. PSI-BLAST, when carried out using ...

A dipeptide of unknown amino acid composition was subjected to acid hydrolysis and heat to permit breakage of peptide bonds in the sample resulting in each individual amino acid constituent. Chromatography was then employed with a proper solvent system to compare a set of known amino acid standards against the unknown hydrolyzed sample to determine the two amino acids present in the unknown dip...

Nucleotide binding and oligomerization domain-containing protein 2 (NOD2/Card15) is an intracellular protein that is involved in the recognition of bacterial cell wall-derived muramyl dipeptide. Mutations in the gene encoding NOD2 are associated with inherited inflammatory disorders, including Crohn disease and Blau syndrome. NOD2 is a member of the nucleotide-binding domain and leucine-rich re...

Running title: SVM-based method for subcellular localization of human proteins SVM-based method for subcellular localization of human proteins 2 Summary Here we report a systematic approach for predicting subcellular localization (cytoplasm, mitochondrial, nuclear and plasma membrane) of human proteins. Firstly, SVM based modules for predicting subcellular localization using traditional amino a...

BACKGROUND Curcumin, the principal curcuminoid of the popular Indian spice turmeric, has a wide spectrum of pharmaceutical properties such as antitumor, antioxidant, antiamyloid, and anti-inflammatory activity. However, poor aqueous solubility and low bioavailability of curcumin is a major challenge in its development as a useful drug. To enhance the aqueous solubility and bioavailability of cu...

Here we report a systematic approach for predicting subcellular localization (cytoplasm, mitochondrial, nuclear, and plasma membrane) of human proteins. First, support vector machine (SVM)-based modules for predicting subcellular localization using traditional amino acid and dipeptide (i + 1) composition achieved overall accuracy of 76.6 and 77.8%, respectively. PSI-BLAST, when carried out usin...

Energy transfer between a naphthalene-dipeptide and either a hosted dansyl acceptor or a co-assembling anthracene-dipeptide acceptor occurs on formation of a hydrogel by decreasing the pH.

Transforming the C-terminal amide of a glycinylphenylalanine dipeptide into N-amidothiourea affords a β-turn structure in the formed dipeptide based N-amidothioureas, which can be readily identified by an induced CD signal from the achiral phenylthiourea chromophore.

Understanding the specific survival of the rare chronic myelogenous leukaemia (CML) stem cell population could provide a target for therapeutics aimed at eradicating these cells. However, little is known about how survival signalling is regulated in CML stem cells. In this study, we survey global metabolic differences between murine normal haematopoietic stem cells (HSCs) and CML stem cells usi...

A quantitative perfusion method has been used to study intestinal absorption of two dipeptides-glycyl-glycine and glycyl-l-alanine-in normal subjects. In each case, the constituent amino acids were absorbed faster when presented as dipeptides than as free amino acids, suggesting intact dipeptide transport. During absorption constituent amino acids were measured within the lumen and it is sugges...