to better understand seed dormancy breaking of bread wheat (triticum aestivum l.rl4137), we examined protein synthesis in dormant and after-ripened seeds.we used six treatments including dormant seed, dormant seed that were subjected to gibberllic acid (ga3) for 10 hours, dormant seed with one, three and six weeks of after-ripening and finally six weeks after-ripened seed subjected to abscisc a...

Many studies of protein expression after traumatic brain injury (TBI) have identified biomarkers for diagnosing or determining the prognosis of TBI. In this study, we searched for additional protein markers of TBI using a fluid perfusion impact device to model TBI in S-D rats. Two-dimensional gel electrophoresis and mass spectrometry were used to identify differentially expressed proteins. Afte...

The acid-extractable proteins of nucleoli of Walker 256 carcinosarcoma, regenerating rat liver, and thioacetamidetreated rat liver were separated into 91, 94, and 92 spots, respectively, by two-dimensional polyacrylamide gel electrophoresis. Although the protein patterns from these different nucleoli have remarkable similarities, some qualitative and quantitative differences were found among th...

background: viscerotropic leishmaniasis caused by leishmania tropica poses a significant prob­lem in the diagnosis and treatment management. since differential gene expression is more im­portant in outcome of the infection, we employed proteomic approach to identify potential pro­teins involved in visceralization of l. tropica . methods: the proteomes profiling of l. tropica isolated from cutan...

egfr is a key molecule in cancer cells. egfr signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. we extracted proteins from astrocytoma (iii and iv) oligodendroglioma(iv) tumors and normal brain tissues and then evaluated the protein purity by bradford test and sp...

Alterations of genomic DNAs in primary hepatocellular carcinomas (HCCs) were examined by restriction landmark genomic scanning (I. Hatada et al., Proc. Natl. Acad. Sci. USA, 88: 9523-9527, 1991) which is a 2-dimensional gel analysis that allows detection of deletion, amplification, or other rearrangements of genomic DNA. Sixteen HCC samples together with their normal counterparts were tested in...

Pinellia ternata is an important traditional Chinese medicinal plant. The growth of P. ternata is sensitive to high temperatures. To gain a better understanding of heat stress responses in P. ternata, we performed a comparative proteomic analysis. P. ternata seedlings were subjected to a temperature of 38 °C and samples were collected 24 h after treatment. Increased relative ion leakage and lip...

A rapid method for the identification of known proteins separated by two-dimensional gel electrophoresis is described in which molecular masses of peptide fragments are used to search a protein sequence database. The peptides are generated by in situ reduction, alkylation, and tryptic digestion of proteins electroblotted from two-dimensional gels. Masses are determined at the subpicomole level ...

We performed proteomic studies on lung cancer cells to elucidate the mechanisms that determine histological phenotype. Thirty lung cancer cell lines with three different histological backgrounds (squamous cell carcinoma, small cell lung carcinoma and adenocarcinoma) were subjected to two-dimensional difference gel electrophoresis (2-D DIGE) and grouped by multivariate analyses on the basis of t...

In order to conduct a comparative proteomics study of human nasopharyngeal carcinoma (NPC) to understand the molecular mechanisms that participate in the formation of NPC, the two-dimensional gel electrophoresis (2-DE) reference map of human NPC tissue proteome was described. To provide a high level of reproducibility between gels and accurately array each protein expressed in NPC tissue proteo...